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PROSITE documentation PDOC00452 [for PROSITE entry PS00522] |
DNA polymerases (EC 2.7.7.7) can be classified, on the basis of sequence similarity [1], into at least four different groups: A, B, C and X. DNA polymerases that belong to family X are listed below [2]:
These enzymes are small (about 40 Kd) compared with other polymerases and their reaction mechanism operates via a distributive mode, i.e. they dissociate from the template-primer after addition of each nucleotide.
As a signature pattern for this family of DNA polymerases, we selected a highly conserved region that contains a conserved arginine and two conserved aspartic acid residues. The latter together with the arginine have been shown [5] to be involved in primer binding in polymerase β.
Last update:May 2004 / Text revised.
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PROSITE method (with tools and information) covered by this documentation:
1 | Authors | Ito J. Braithwaite D.K. |
Title | Compilation and alignment of DNA polymerase sequences. | |
Source | Nucleic Acids Res. 19:4045-4057(1991). | |
PubMed ID | 1870963 |
2 | Authors | Matsukage A. Nishikawa K. Ooi T. Seto Y. Yamaguchi M. |
Title | Homology between mammalian DNA polymerase beta and terminal deoxynucleotidyltransferase. | |
Source | J. Biol. Chem. 262:8960-8962(1987). | |
PubMed ID | 3597402 |
3 | Authors | Prasad R. Widen S.G. Singhal R.K. Watkins J. Prakash L. Wilson S.H. |
Title | Yeast open reading frame YCR14C encodes a DNA beta-polymerase-like enzyme. | |
Source | Nucleic Acids Res. 21:5301-5307(1993). | |
PubMed ID | 8265341 |
4 | Authors | Yanez R.J. Rodriguez J.M. Nogal M.L. Yuste L. Enriquez C. Rodriguez J.F. Vinuela E. |
Title | Analysis of the complete nucleotide sequence of African swine fever virus. | |
Source | Virology 208:249-278(1995). | |
PubMed ID | 11831707 |
5 | Authors | Date T. Yamamoto S. Tanihara K. Nishimoto Y. Matsukage A. |
Title | Aspartic acid residues at positions 190 and 192 of rat DNA polymerase beta are involved in primer binding. | |
Source | Biochemistry 30:5286-5292(1991). | |
PubMed ID | 2036395 |