Home  |  Contact
PROSITE documentation PDOC00535 [for PROSITE entry PS00612]

Osteonectin domain signatures





Description

The following extracellular proteins contain, in their C-terminal section, a domain of about 240 amino acids which is highly conserved [1]. These proteins are:

  • SPARC (also known as Osteonectin or BM-40), a glycoprotein that appears to regulate cell growth through interactions with the extracellular matrix and cytokines. It binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; a acidic domain that binds 5 to 8 Ca++ with a low affinity and a EF- hand loop that binds a Ca++ ion with a high affinity. SPARC is expressed at high levels in tissues undergoing morphogenesis, remodeling and repair.
  • SC1, a mammalian brain extracellular matrix protein.
  • QR1, an avian protein, specifically expressed in the neuroretina.

This domain has a N-terminal section of about 90 residues that contains 11 conserved cysteines, a central section that is probably in an α-helical conformation, and a C-terminal section that contains two EF-hand type calcium-binding regions [2] and three conserved cysteines. This topology is schematically represented below.

                                                                     +----+
                                                                     |    |
 xCxCxCxxCCxCxxxCxxCxCxxxxxCxCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx<Ca>xCxxC<Ca>Cxx
  ***********                   ***
'C' : conserved cysteines involved in disulfide bonds.
'Ca': EF-hand region (see <PDOC00018>).
'*' : position of the patterns.

We developed two signature patterns for this domain. One is located in the C-terminal cysteine-rich section, the other consists in a highly conserved stretch of 11 residues in the central section.

Expert(s) to contact by email:

Maurer P.

Last update:

December 2004 / Pattern and text revised.

Technical section

PROSITE methods (with tools and information) covered by this documentation:

OSTEONECTIN_1, PS00612; Osteonectin domain signature 1  (PATTERN)

OSTEONECTIN_2, PS00613; Osteonectin domain signature 2  (PATTERN)


References

1AuthorsLane T.F. Sage E.H.
TitleThe biology of SPARC, a protein that modulates cell-matrix interactions.
SourceFASEB J. 8:163-173(1994).
PubMed ID8119487

2AuthorsHohenester E. Maurer P. Hohenadl C. Timpl R. Jansonius J.N. Engel J.
TitleStructure of a novel extracellular Ca(2+)-binding module in BM-40.
SourceNat. Struct. Biol. 3:67-73(1996).
PubMed ID8548457



PROSITE is copyright. It is produced by the SIB Swiss Institute Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to
Prosite License or see: prosite_license.html.

Miscellaneous

View entry in original PROSITE document format
View entry in raw text format (no links)