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PROSITE documentation PDOC00527 [for PROSITE entry PS00742] |
A number of enzymes that catalyze the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) via a phospho-histidine intermediate have been shown to be structurally related [1,2,3,4]. These enzymes are:
All these enzymes share the same catalytic mechanism: they bind PEP and transfer the phosphoryl group from it to a histidine residue. The sequence around that residue is highly conserved and can be used as a signature pattern for these enzymes. As a second signature pattern we selected a conserved region in the C-terminal part of the PEP-utilizing enzymes. The biological significance of this region is not yet known.
Last update:December 2004 / Pattern and text revised.
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PROSITE methods (with tools and information) covered by this documentation:
1 | Authors | Reizer J. Hoischen C. Reizer A. Pham T.N. Saier M.H. Jr. |
Title | Sequence analyses and evolutionary relationships among the energy-coupling proteins Enzyme I and HPr of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. | |
Source | Protein Sci. 2:506-521(1993). | |
PubMed ID | 7686067 |
2 | Authors | Reizer J. Reizer A. Merrick M.J. Plunkett G. III Rose D.J. Saier M.H. Jr. |
Title | Novel phosphotransferase-encoding genes revealed by analysis of the Escherichia coli genome: a chimeric gene encoding an Enzyme I homologue that possesses a putative sensory transduction domain. | |
Source | Gene 181:103-108(1996). | |
PubMed ID | 8973315 |
3 | Authors | Pocalyko D.J. Carroll L.J. Martin B.M. Babbitt P.C. Dunaway-Mariano D. |
Title | Analysis of sequence homologies in plant and bacterial pyruvate phosphate dikinase, enzyme I of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and other PEP-utilizing enzymes. Identification of potential catalytic and regulatory motifs. | |
Source | Biochemistry 29:10757-10765(1990). | |
PubMed ID | 2176881 |
4 | Authors | Niersbach M. Kreuzaler F. Geerse R.H. Postma P.W. Hirsch H.J. |
Title | Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase. | |
Source | Mol. Gen. Genet. 231:332-336(1992). | |
PubMed ID | 1310524 |