|PROSITE documentation PDOC00711 [for PROSITE entry PS00918]|
Deoxyribonuclease I (DNase I) (EC 18.104.22.168)  is a vertebrate enzyme which catalyzes the endonucleolytic cleavage of double-stranded DNA to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products. DNase I is an enzyme involved in DNA degradation; it is normally secreted outside of the cell but seems to be able to gain access to the nucleus where it is involved in cell death by apoptosis .
As shown in the following schematic representation, DNase I is a glycoprotein of about 260 residues with two conserved disulfide bonds.
+-+ +--------+ | | | | xxxxxxxxxxxxxxxxx#xxxxxxCxCxxxxx#xxxxxxxxxCxxxxxxxxCxxxxxxxxxxxxx **** ****
'C': conserved cysteine involved in a disulfide bond. '#': active site residue. '*': position of the patterns.
DNase I has a pH-optimum around 7.5 and requires calcium and magnesium for full activity. It causes single strand nicks in duplex DNA. A proton acceptor-donor chain composed of an histidine and a glutamic acid produce a nucleophilic hydroxyl ion from water, which cleaves the 3'-P-O bond . DNase I is inhibited by actin with which it forms a stoechiometric 1:1 complex .
The sequence of DNase I is evolutionary related to that of:
The first disulfide bond of DNase I is not conserved in the above proteins.Last update:
April 2006 / Pattern revised.
PROSITE methods (with tools and information) covered by this documentation:
|1||Authors||Suck D. Oefner C.|
|Title||Structure of DNase I at 2.0 A resolution suggests a mechanism for binding to and cutting DNA.|
|2||Authors||Peitsch M.C. Polzar B. Stephan H. Crompton T. MacDonald H.R. Mannherz H.G. Tschopp J.|
|Title||Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death).|
|Source||EMBO J. 12:371-377(1993).|
|3||Authors||Suck D. Lahm A. Oefner C.|
|Title||Structure refined to 2A of a nicked DNA octanucleotide complex with DNase I.|
|4||Authors||Kabsch W. Mannherz H.G. Suck D. Pai E.F. Holmes K.C.|
|Title||Atomic structure of the actin:DNase I complex.|
|5||Authors||Parrish J.E. Ciccodicola A. Wehhert M. Cox G.F. Chen E. Nelson D.L.|
|Title||A muscle-specific DNase I-like gene in human Xq28.|
|Source||Hum. Mol. Genet. 4:1557-1564(1995).|
|6||Authors||Rodriguez A.M. Rodin D. Nomura H. Morton C.C. Weremowicz S. Schneider M.C.|
|Title||Identification, localization, and expression of two novel human genes similar to deoxyribonuclease I.|