|PROSITE documentation PDOC00754 [for PROSITE entry PS00981]|
Glutamate and calcium bind to G-protein coupled receptors that, while structurally similar to the majority of G-protein coupled receptors (R7G) (see the relevant entry <PDOC00210>), do not show any similarity at the level of their sequence, thus representing a new family whose current known members are listed below:
Structurally these receptors are composed of:
a) A signal sequence; b) A very large hydrophilic extracellular region of about 540 to 600 amino acid residues. This region contains 17 conserved cysteines which could be involved in disulfide bonds; c) A region of about 250 residues that seem to contain seven transmembrane domains; d) A C-terminal cytoplasmic domain of variable length (50 to 350 residues).
There are quite a number of regions of high sequence conservation both in the N-terminal domain and in the region containing the transmembrane domains. We have selected three of these conserved regions as signature patterns. The first one corresponds to a highly conserved hydrophobic segment in the central part of the N-terminal extracellular region. The second corresponds to a section that contains a cluster of six cysteines in the C-terminal part of the extracellular domain. The last one corresponds to the C-terminal part of the cytoplasmic loop between the fifth and sixth transmembrane domains.
We also developed a profile which covers the seven transmembrane regions.Expert(s) to contact by email:
April 2006 / Patterns revised.
PROSITE methods (with tools and information) covered by this documentation:
|1||Authors||Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.|
|Title||A family of metabotropic glutamate receptors.|
|2||Authors||Okamoto N., Hori S., Akazawa C., Hayashi Y., Shigemoto R., Mizuno N., Nakanishi S.|
|Title||Molecular characterization of a new metabotropic glutamate receptor mGluR7 coupled to inhibitory cyclic AMP signal transduction.|
|Source||J. Biol. Chem. 269:1231-1236(1994).|
|3||Authors||Duvoisin R.M., Zhang C., Ramonell K.|
|Title||A novel metabotropic glutamate receptor expressed in the retina and olfactory bulb.|
|Source||J. Neurosci. 15:3075-3083(1995).|
|4||Authors||Brown E.M., Gamba G., Riccardi D., Lombardi M., Butters R., Kifor O., Sun A., Hediger M.A., Lytton J., Hebert S.C.|
|Title||Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid.|