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	PROSITE documentation PDOC00885 [for PROSITE entry PS01149]

Rsu family of pseudouridine synthase signature

The following bacterial proteins, which seems to belong to a family of pseudouridine synthases (EC 5.4.99.-) [1] have been shown to share regions of similarities:

• Escherichia coli and Haemophilus influenzae 16S pseudouridylate 516 synthase (EC 5.4.99.-) (gene: rsuA). This enzyme is responsible for the formation of pseudouridine from uracil-516 in 16S ribosomal RNA.
• Escherichia coli and Haemophilus influenzae ribosomal large subunit pseudouridine synthase B (rluB).
• Bacillus subtilis ribosomal large subunit pseudouridine synthase B (rluB).
• Escherichia coli ribosomal large subunit pseudouridine synthase F (rluF).
• Escherichia coli and Haemophilus influenzae ribosomal large subunit pseudouridine synthase E (rluE).
• Aquifex aeolicus hypothetical protein AQ_554.
• Aquifex aeolicus hypothetical protein AQ_1464.
• Bacillus subtilis hypothetical protein ytzF.
• Borrelia burgdorferi hypothetical protein BB0129.
• Helicobacter pylori hypothetical protein HP1459.
• Synechocystis strain PCC 6803 hypothetical protein slr0361.
• Synechocystis strain PCC 6803 hypothetical protein slr0612.

These are proteins of from 25 to 40 Kd which contain a number of conserved regions in their central section. They can be picked up in the database by the following highly conserved pattern.

Last update:

April 2005 / Text revised.

PROSITE method (with tools and information) covered by this documentation:

PSI_RSU, PS01149; Rsu family of pseudouridine synthase signature  (PATTERN)

• Consensus pattern:
  G-R-L-D-x(2)-[STA]-x-G-[LIVFA]-[LIVMF](3)-[ST]-[DNST]
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 73
  • detected by PS01149: 73 (true positives)
  • undetected by PS01149: 0 (false negative or 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01149:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01149
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01149
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01149
• View ligand binding statistics of PS01149
• Matching PDB structures: 1VIO 2GML 2OLW 2OML ... [ALL]

1	Authors	Wrzesinski J., Bakin A., Nurse K., Lane B.G., Ofengand J.
	Title	Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli.
	Source	Biochemistry 34:8904-8913(1995).
	PubMed ID	7612632

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