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	PROSITE documentation PDOC00952 [for PROSITE entry PS01238]

GDA1/CD39 family of nucleoside phosphatases signature

A number of nucleoside diphosphate and triphosphate hydrolases as well as some yet uncharacterized proteins have been found to belong to the same family [1,2]. This family currently consist of:

• Yeast guanosine-diphosphatase (EC 3.6.1.42) (GDPase) (gene GDA1). GDA1 is a golgi integral membrane enzyme that catalyzes the hydrolysis of GDP to GMP.
• Potato apyrase (EC 3.6.1.5) (adenosine diphosphatase) (ADPase). Apyrase acts on both ATP and ADP to produce AMP.
• Mammalian vascular ATP-diphosphohydrolase (EC 3.6.1.5) (also known as lymphoid cell activation antigen CD39).
• Toxoplasma gondii nucleoside-triphosphatases (EC 3.6.1.15) (NTPase). NTPase hydrolyses various nucleoside triphosphates to produce the corresponding nucleoside mono- and diphosphates. This enzyme is secreted into the invaded host cell into the parasitophorous vacuole, a specialized compartment where the parasite intracellulary resides.
• Pea nucleoside-triphosphatases (EC 3.6.1.15) (NTPase).
• Caenorhabditis elegans hypothetical protein C33H5.14.
• Caenorhabditis elegans hypothetical protein R07E4.4.
• Yeast chromosome V hypothetical protein YER005w.

The above uncharacterized proteins all seem to be membrane-bound.

All these proteins share a number of conserved domains. We have selected the best conserved of these domains. It is located in the central section of the proteins.

Last update:

December 2004 / Pattern and text revised.

PROSITE method (with tools and information) covered by this documentation:

GDA1_CD39_NTPASE, PS01238; GDA1/CD39 family of nucleoside phosphatases signature  (PATTERN)

• Consensus pattern:
  [LIVM]-x-G-x(2)-E-G-x-[FYLS]-x-[FW]-[LIVA]-[TAG]-x-N-[HYF]
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 55
  • detected by PS01238: 50 (true positives)
  • undetected by PS01238: 5 (5 false negatives and 0 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01238:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01238
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01238
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01238
• View ligand binding statistics of PS01238
• Matching PDB structures: 3CJ1 3CJ7 3CJ9 3CJA ... [ALL]

1	Authors	Handa M., Guidotti G.
	Title	Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).
	Source	Biochem. Biophys. Res. Commun. 218:916-923(1996).
	PubMed ID	8579614

2	Authors	Vasconcelos E.G., Ferreira S.T., de Carvalho T.M.U., de Souza W., Kettlun A.M., Mancilla M., Valenzuela M.A., Verjovski-Almeida S.
	Title	Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni. Immunological cross-reactivities with potato apyrase and Toxoplasma gondii nucleoside triphosphate hydrolase.
	Source	J. Biol. Chem. 271:22139-22145(1996).
	PubMed ID	8703025

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