|PROSITE documentation PDOC50003 [for PROSITE entry PS50003]|
The 'pleckstrin homology' (PH) domain is a domain of about 100 residues that occurs in a wide range of proteins involved in intracellular signaling or as constituents of the cytoskeleton [1,2,3,4,5,6,7].
The function of this domain is not clear, several putative functions have been suggested:
It is possible that different PH domains have totally different ligand requirements.
The 3D structure of several PH domains has been determined . All known cases have a common structure consisting of two perpendicular anti-parallel β sheets, followed by a C-terminal amphipathic helix. The loops connecting the β-strands differ greatly in length, making the PH domain relatively difficult to detect. There are no totally invariant residues within the PH domain.
Proteins reported to contain one more PH domains belong to the following families:
The profile for the PH domain, which has been developed by Toby Gibson at the EMBL, covers the total length of domain. Several proteins contain large insertions in the PH domain and are thus difficult to detect with this profile. In some of these cases, the profile will align only to one half of the PH domain.Expert(s) to contact by email:
March 2002 / Text revised.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Mayer B.J. Ren R. Clark K.L. Baltimore D.|
|Title||A putative modular domain present in diverse signaling proteins.|
|2||Authors||Haslam R.J. Koide H.B. Hemmings B.A.|
|Title||Pleckstrin domain homology.|
|3||Authors||Musacchio A. Gibson T.J. Rice P. Thompson J. Saraste M.|
|Title||The PH domain: a common piece in the structural patchwork of signalling proteins.|
|Source||Trends Biochem. Sci. 18:343-348(1993).|
|4||Authors||Gibson T.J. Hyvonen M. Musacchio A. Saraste M. Birney E.|
|Title||PH domain: the first anniversary.|
|Source||Trends Biochem. Sci. 19:349-353(1994).|
|Title||Protein modules and signalling networks.|
|6||Authors||Ingley E. Hemmings B.A.|
|Title||Pleckstrin homology (PH) domains in signal transduction.|
|Source||J. Cell. Biochem. 56:436-443(1994).|
|7||Authors||Saraste M. Hyvonen M.|
|Title||Pleckstrin homology domains: a fact file.|
|Source||Curr. Opin. Struct. Biol. 5:403-408(1995).|
|Title||More meanders and sandwiches.|
|Source||Nat. Struct. Biol. 1:755-757(1994).|