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PROSITE documentation PDOC50172 [for PROSITE entry PS50172]

BRCT domain profile





Description

The breast cancer susceptibility gene contains at its C-terminus two copies of a conserved domain that was named BRCT for BRCA1 C-terminus. This domain of about 95 amino acids is found in a large variety of proteins involved in DNA-repair, recombination and cell cycle control [1,2,3]. The BRCT domain is not limited to the C-termini of protein sequences and can be found in multiple copies or in a single copy as in RAP1 and TdT. Recent data [4] indicate that the BRCT domain functions as a protein-protein interaction module.

The structure of the first of the two C-terminal BRCT domains of the human DNA repair protein XRCC1 has recently been determined by X-ray crystallography [4].

Some proteins known to contain a BRCT domain are listed below:

  • Mammalian breast cancer type 1 susceptibility protein. It may regulate gene expression.
  • Human p53-binding protein 1.
  • Human poly(ADP-ribose) polymerase (PARP) (EC 2.4.2.30). It modifies various nuclear proteins by poly(ADP-rybosyl)ation.
  • Vertebrate DNA nucleotidylexotransferase (EC 2.7.7.31). It adds nucleotides at the junction (N region) of rearranged Ig heavy chain and T cell receptor gene segments during the maturation of B and T cells.
  • Mammalian DNA-repair protein XRCC1.
  • Human DNA ligase III (EC 6.5.1.1). It is involved in DNA strand-break repair.
  • Human DNA ligase IV (EC 6.5.1.1).
  • Drosophila germline transcription factor 1. A putative transcription factor active during oogenesis and embryogenesis.
  • Baker's yeast DNA ligase II (EC 6.5.1.1).
  • Baker's yeast RAD9 protein. It is essential for cell cycle arrest following DNA damage by X-irradiation or inactivation of DNA ligase.
  • Baker's yeast RAP1. It is involved in telomeric and HM loci silencing.
  • Escherichia coli DNA ligase (EC 6.5.1.2). It is essential for DNA replication and repair of damaged DNA.
  • Synechocystis sp. DNA ligase (EC 6.5.1.2). It is probably essential for DNA replication and repair of damaged DNA.
Expert(s) to contact by email:

Hofmann K.

Last update:

December 2001 / First entry.

Technical section

PROSITE method (with tools and information) covered by this documentation:

BRCT, PS50172; BRCT domain profile  (MATRIX)


References

1AuthorsKoonin E.V. Altschul S.F. Bork P.
TitleBRCA1 protein products ... Functional motifs...
SourceNat. Genet. 13:266-268(1996).
PubMed ID8673121
DOI10.1038/ng0796-266

2AuthorsBork P. Hofmann K. Bucher P. Neuwald A.F. Altschul S.F. Koonin E.V.
TitleA superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins.
SourceFASEB J. 11:68-76(1997).
PubMed ID9034168

3AuthorsCallebaut I. Mornon J.-P.
TitleFrom BRCA1 to RAP1: a widespread BRCT module closely associated with DNA repair.
SourceFEBS Lett. 400:25-30(1997).
PubMed ID9000507

4AuthorsZhang X. Morera S. Bates P.A. Whitehead P.C. Coffer A.I. Hainbucher K. Nash R.A. Sternberg M.J. Lindahl T. Freemont P.S.
TitleStructure of an XRCC1 BRCT domain: a new protein-protein interaction module.
SourceEMBO J. 17:6404-6411(1998).
PubMed ID9799248
DOI10.1093/emboj/17.21.6404



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