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PROSITE documentation PDOC50250 [for PROSITE entry PS50250]

PCI domain profile





Description

The PCI (for Proteasome, COP9, Initiation factor 3) domain (sometimes also referred to as the PINT domain, for Proteasome subunits, Int-6, Nip-1, and Trip-15) is present in six different subunits of 26 proteasome lid, COP9 signalosome (CSN) and eukaryotic translation initiation factor-3 (eIF3) complexes, as well as in subunits of certain other multiprotein complexes. The PCI domain mediates and stabilizes protein-protein interactions within the complexes. The role of the PCI domains is most likely that of a scaffold for the other complex subunits and other binding partners. The PCI domain could play a role as a universal binding domain supporting intra-complex interactions as well as recruitments of additional ligands [1,2,3,4,5,6].

PCI is an ~190-amino acid domain, not well conserved in its primary sequence, usually located near the C-terminus of the protein. It does not contain any invariant residues or any conserved pattern of charged residues that would suggest a catalytic activity. The PCI domain is comprised of two subdomains that are intimately connected, an N-terminal helical bundle (HB) subdomain and a C-terminal globular winged helix (WH) subdomain (see <PDB:3CHM>). The C-terminal half of the PCI domain is much better conserved than the N-terminal halfdomain [4,5,6].

Some proteins known to contain a PCI domain are listed below:

  • Eukaryotic 26S proteasome non-ATPase regulatory subunit 3 (PSMD3).
  • Eukaryotic 26S proteasome non-ATPase regulatory subunit 6 (PSMD6).
  • Eukaryotic 26S proteasome non-ATPase regulatory subunit 8 (PSMD8).
  • Eukaryotic 26S proteasome non-ATPase regulatory subunit 11 (PSMD11)
  • Eukaryotic 26S proteasome non-ATPase regulatory subunit 12 (PSMD12).
  • Eukaryotic 26S proteasome non-ATPase regulatory subunit 13 (PSMD13).
  • Eukaryotic COP9 signalosome complex subunit 1 (CSN1).
  • Eukaryotic COP9 signalosome complex subunit 2 (CSN2).
  • Eukaryotic COP9 signalosome complex subunit 3 (CSN3).
  • Eukaryotic COP9 signalosome complex subunit 4 (CSN4).
  • Eukaryotic COP9 signalosome complex subunit 7 (CSN7).
  • Eukaryotic COP9 signalosome complex subunit 8 (CSN8).
  • Eukaryotic translation initiation factor 3 subunit A (EIF3A).
  • Eukaryotic translation initiation factor 3 subunit C (EIF3C).
  • Eukaryotic translation initiation factor 3 subunit E (EIF3E).
  • Eukaryotic translation initiation factor 3 subunit K (EIF3K).
  • Eukaryotic translation initiation factor 3 subunit L (EIF3L).
  • Eukaryotic translation initiation factor 3 subunit M (EIF3M).
  • Eukaryotic PCI domain-containing protein 2 (PCID2), a component of the transcription and export complex 2 (TREX-2 complex) that functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket).
  • Eukaryotic germinal-center associated nuclear protein (GANP), a component of the transcription and export complex 2 (TREX-2 complex) that functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket).

The profile we developed covers the entire PCI domain.

Last update:

July 2017 / First entry.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

PCI, PS50250; PCI domain profile  (MATRIX)


References

1AuthorsHofmann K. Bucher P.
TitleThe PCI domain: a common theme in three multiprotein complexes.
SourceTrends Biochem. Sci. 23:204-205(1998).
PubMed ID9644972

2AuthorsAravind L. Ponting C.P.
TitleHomologues of 26S proteasome subunits are regulators of transcription and translation.
SourceProtein Sci. 7:1250-1254(1998).
PubMed ID9605331
DOI10.1002/pro.5560070521

3AuthorsScheel H. Hofmann K.
TitlePrediction of a common structural scaffold for proteasome lid, COP9-signalosome and eIF3 complexes.
SourceBMC Bioinformatics. 6:71-71(2005).
PubMed ID15790418
DOI10.1186/1471-2105-6-71

4AuthorsLee J.-H. Yi L. Li J. Schweitzer K. Borgmann M. Naumann M. Wu H.
TitleCrystal structure and versatile functional roles of the COP9 signalosome subunit 1.
SourceProc. Natl. Acad. Sci. U. S. A. 110:11845-11850(2013).
PubMed ID23818606
DOI10.1073/pnas.1302418110

5AuthorsWei Z. Zhang P. Zhou Z. Cheng Z. Wan M. Gong W.
TitleCrystal structure of human eIF3k, the first structure of eIF3 subunits.
SourceJ. Biol. Chem. 279:34983-34990(2004).
PubMed ID15180986
DOI10.1074/jbc.M405158200

6AuthorsDessau M. Halimi Y. Erez T. Chomsky-Hecht O. Chamovitz D.A. Hirsch J.A.
TitleThe Arabidopsis COP9 signalosome subunit 7 is a model PCI domain protein with subdomains involved in COP9 signalosome assembly.
SourcePlant Cell 20:2815-2834(2008).
PubMed ID18854373
DOI10.1105/tpc.107.053801



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