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PROSITE documentation PDOC00694 [for PROSITE entry PS51084]

HIT domain signature and profile





Description

HIT (histidine triad) proteins, named for a short motif related to the sequence (His-x-His-x-His-x-x, where x is a hydrophobic residue) [1,2] are a superfamily of nucleotide hydrolases which acts on the α-phosphate of ribonucleotides [3,4]. This superfamily can be divided in two branches: the Hint branch that is the most widely conserved and has representatives in all cellular life and the Fhit (for ôfragile histidine triadö) branch which is only found in animals and fungi. The two branches share a conserved core region of about 1oo amino acids, the HIT domain.

Crystal structures of the HINT-nucleotide complexe revealed that the HIT domain is related to GalT nucleotide-binding proteins (see <PDOC00108>) (see <PDB:3RHN>) [3]. The GalT monomer can be seen as consisting of two repeated HIT domains. Their nucleotide binding sites also retain some of the same residues for binding a nucleoside monophosphate. The histidine triad is located outside the nucleotide binding pocket and stabilize it.

Some proteins known to contain a HIT domain are listed below:

As a signature pattern, we selected the region of the histidine triad. We also developed a profile that covers the whole HIT domain.

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html --------------------------------------------------------------------------------.

Expert(s) to contact by email:

Seraphin B.

Aitken A.

Brenner C.

Last update:

April 2006 / Pattern revised.

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Technical section

PROSITE methods (with tools and information) covered by this documentation:

?, PS51084´┐;   (?)

HIT_1, PS00892; HIT domain signature  (PATTERN)


Reference



PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

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