|PROSITE documentation PDOC51230 [for PROSITE entry PS51230]|
A group of microtubule-associated proteins called +TIPs (plus end tracking proteins), including EB1 (end-binding protein 1) family proteins, label growing microtubules ends specifically in diverse organisms and are implicated in spindle dynamics, chromosome segregation, and directing microtubules toward cortical sites. EB1 members have a bipartite composition: the N-terminal CH domain (see <PDOC50021>) mediates microtubule plus end localization and a C-terminal cargo binding domain (EB1-C) that captures cell polarity determinants. The EB1-C domain comprises a unique EB1-like sequence motif that acts as a binding site for other +TIP proteins. It interacts with the carboxy terminus of the adenomatous polyposis coli (APC) tumor suppressor, a well conserved +TIP phosphoprotein with a pivotal function in cell cycle regulation. Another binding partner of the EB1-C domain is the well conserved +TIP protein dynactin, a component of the large cytoplasmic dynein/dynactin complex [1,2,3].
The ~80-residue EB1-C domain starts with a long smoothly curved helix (α1), which is followed by a hairpin connection leading to a short second helix (α2) running antiparallel to α1 (see <PDB:1WU9>). The two parallel α1 helices of the EB1-C domain dimer wrap around each other in a slightly left-handed supercoil. The two α2 helices run antiparallel to helices α1 and form a similar fork in the opposite orientation and rotated by 90°. As a result, two helical segments from each monomer form a four-helix bundle. The side chain forming the hydrophobic core of this bundle are highly conserved [2,3,4].
Some protein known to contain a EB1-C domain are listed below:
The profile we developed covers the entire EB1-C domain.Last update:
July 2006 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Mathur J. Mathur N. Kernebeck B. Srinivas B.P. Huelskamp M.|
|Title||A novel localization pattern for an EB1-like protein links microtubule dynamics to endomembrane organization.|
|Source||Curr. Biol. 13:1991-1997(2003).|
|2||Authors||Slep K.C. Rogers S.L. Elliott S.L. Ohkura H. Kolodziej P.A. Vale R.D.|
|Title||Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the microtubule plus end.|
|Source||J. Cell Biol. 168:587-598(2005).|
|3||Authors||Honnappa S. John C.M. Kostrewa D. Winkler F.K. Steinmetz M.O.|
|Title||Structural insights into the EB1-APC interaction.|
|Source||EMBO J. 24:261-269(2005).|
|4||Authors||Hayashi I. Wilde A. Mal T.K. Ikura M.|
|Title||Structural basis for the activation of microtubule assembly by the EB1 and p150Glued complex.|
|Source||Mol. Cell 19:449-460(2005).|