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PROSITE documentation PDOC51338 [for PROSITE entry PS51338]

IMD domain profile





Description

The N-terminal IRSp53 and MIM homology domain (IMD) is a BAR-like domain of ~250 amino acids that is conserved in an evolutionarily related protein family, the IRSp53/MIM family. The IRSp53/MIM family is a novel F-actin bundling protein family that includes invertebrate relatives:

  • Vertebrate missing in metastasis (MIM), an actin-binding scaffold protein that may be involved in cancer metastasis.
  • Vertebrate ABBA-1, a MIM-related protein.
  • Vertebrate brain-specific angiogenesis inhibitor 1-associated protein 2 (BAI1-associated protein 2) or insulin receptor tyrosine kinase substrate p53 (IRSp53), a multifunctional adaptor protein that links Rac1 with a Wiskott-Aldrich syndrome family verprolin-homologous protein 2 (WAVE2) to induce lamellipodia or Cdc42 with Mena to induce filopodia.
  • Vertebrate brain-specific angiogenesis inhibitor 1-associated protein 2- like proteins 1 and 2 (BAI1-associated protein 2-like proteins 1 and 2).
  • Drosophila melanogaster CG32082-PA.
  • Caenorhabditis elegans M04F3.5 protein.

The vertebrate IRSp53/MIM family is divided into two major groups: the IRSp53 subfamily and the MIM/ABBA subfamily. The putative invertebrate homologs are positioned between them. The IRSp53 subfamily members contain an SH3 domain (see <PDOC50002>), and the MIM/ABBA subfamily proteins contain a WH2 domain (see <PDOC51082>). The vertebrate SH3-containing subfamily is further divided into three groups according to the presence or absence of the WWB and the half-CRIB motif. The IMD domain can bind to and bundle actin filaments, bind to membranes and interact with the small GTPase Rac [1,2].

The IMD domain folds as a coiled coil of three extended α-helices and a shorter C-terminal helix (see <PDB:1Y2O>). Helix 4 packs tightly against the other three helices, and thus represents an integral part of the domain. The fold of the IMD domain closely resembles that of the BAR (Bin-Amphiphysin-RVS) domain (see <PDOC51021>), a functional module serving both as a sensor and inducer of membrane curvature [3].

The profile we developed covers the entire IMD domain.

Last update:

November 2007 / First entry.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

IMD, PS51338; IMD domain profile  (MATRIX)


References

1AuthorsYamagishi A. Masuda M. Ohki T. Onishi H. Mochizuki N.
TitleA novel actin bundling/filopodium-forming domain conserved in insulin receptor tyrosine kinase substrate p53 and missing in metastasis protein.
SourceJ. Biol. Chem. 279:14929-14936(2004).
PubMed ID14752106
DOI10.1074/jbc.M309408200

2AuthorsMachesky L.M. Johnston S.A.
TitleMIM: a multifunctional scaffold protein.
SourceJ. Mol. Med. 85:569-576(2007).
PubMed ID17497115
DOI10.1007/s00109-007-0207-0

3AuthorsMillard T.H. Bompard G. Heung M.Y. Dafforn T.R. Scott D.J. Machesky L.M. Fuetterer K.
TitleStructural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53.
SourceEMBO J. 24:240-250(2005).
PubMed ID15635447
DOI10.1038/sj.emboj.7600535



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