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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00204


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00204
General rule information [?]

Accession PRU00204
Dates 12-DEC-2003 (Created)
03-SEP-2024 (Last updated, Version 13)
Data class Domain;
Predictors PROSITE; PS50104; TIR
Name TIR
Function The TIR domain has been defined as a scaffold that promotes assembly of signaling complexes via protein-protein interactions. However, the scaffolding function may be a a recent adaptation. The primordial function of the TIR domain is a self-association-dependent nicotinamide dinucleotide (NAD(+))-cleaving enzyme (NADase) activity that cleaves NAD(+) into nicotinamide (Nam) and ADP-ribose (ADPR), cyclic ADPR (cADPR) or variant cADPR (v-cADPR), with catalytic cleavage executed by a conserved glutamic acid.
Scope(s) Eukaryota
Example(s) Q6SZW1;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <Feature:PS50104:6=F> and <Feature:PS50104:10=R> and <Feature:PS50104:36=D> and <Feature:PS50104:50=L> and <Feature:PS50104:73=W> and <Feature:PS50104:77=E>
Protein name + AltName: Full=Probable NAD(+) hydrolase;
                 EC=3.2.2.6;

Comments [?]

CATALYTIC ACTIVITY Reaction=NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+); Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
DOMAINThe TIR domain mediates NAD(+) hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

Keywords [?]

NAD
Hydrolase
end case

Features [?]

From: PS50104
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="TIR #"
ACT_SITE 77 77 E
case <Feature:PS50104:6=F> and <Feature:PS50104:10=R> and <Feature:PS50104:36=D> and <Feature:PS50104:50=L> and <Feature:PS50104:73=W>
BINDING 10 11 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
BINDING 41 41 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 E
end case

Additional information [?]

Size range 125-180 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]