ProRule PRU00661
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00661
General rule information
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| Accession | PRU00661 |
| Dates | 14-APR-2009 (Created)
03-SEP-2024 (Last updated, Version 16) |
| Data class | Domain; |
| Predictors |
PROSITE; PS51331; THYX |
Name | Flavin-dependent thymidylate synthase (thyX) domain |
| Function | The thyx domain contains FAD and methylates dUMP at position 5 of the pyrimidine ring. |
| Scope(s) |
Bacteria Archaea Viruses Eukaryota Dictyostelium |
| Example(s) | O86840 (THYX_STRCO); |
Propagated annotation
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Identifier, protein and gene names
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| case not <Triggered> | |
| Protein name | AltName: Full=Probable thymidylate synthase; Short=TS; Short=TSase; EC=2.1.1.148; |
| end case | |
Comments
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| FUNCTION | Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant. |
| CATALYTIC ACTIVITY | Reaction=dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+); Xref=Rhea:RHEA:29043, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422; EC=2.1.1.148; |
| COFACTOR | Name=FAD; Xref=ChEBI:CHEBI:57692; synthase) domain |
Keywords
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Gene Ontology
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| GO:0008168; Molecular function:methyltransferase activity |
| GO:0016740; Molecular function:transferase activity |
| GO:0050797; Molecular function:thymidylate synthase (FAD) activity |
| GO:0006231; Biological process:dTMP biosynthetic process |
| GO:0050660; Molecular function:flavin adenine dinucleotide binding |
Features
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| From: PS51331 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="ThyX #" | |||||||||
| MOTIF | 80 | 90 | /note="ThyX motif #" | R-H-R-x(7,8)-S | ||||||||
Additional information
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| Size range | 183-266 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1-2 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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