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ProRule PRU00661


General rule information [?]

PURL https://purl.expasy.org/prosite/rule/PRU00661
Accession PRU00661
Dates 14-APR-2009 (Created)
3-SEP-2024 (Last updated, Version 15)
Data class Domain;
Predictors PROSITE; PS51331; THYX
Name Flavin-dependent thymidylate synthase (thyX) domain
Function The thyx domain contains FAD and methylates dUMP at position 5 of the pyrimidine ring.
Scope(s) Bacteria
Archaea
Viruses
Eukaryota
Dictyostelium
Example(s) O86840 (THYX_STRCO);

Propagated annotation [?]

Identifier, protein and gene names [?]

case not <Triggered>
Protein name AltName: Full=Probable thymidylate synthase;
                 Short=TS;
                 Short=TSase;
                 EC=2.1.1.148;
end case

Comments [?]

FUNCTIONCatalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.
CATALYTIC ACTIVITY Reaction=dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+); Xref=Rhea:RHEA:29043, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422; EC=2.1.1.148;
COFACTOR Name=FAD; Xref=ChEBI:CHEBI:57692; synthase) domain

Keywords [?]

FAD
Flavoprotein
Methyltransferase
Nucleotide biosynthesis
Transferase

Gene Ontology [?]

GO:0008168; Molecular function:methyltransferase activity
GO:0016740; Molecular function:transferase activity
GO:0050797; Molecular function:thymidylate synthase (FAD) activity
GO:0006231; Biological process:dTMP biosynthetic process
GO:0050660; Molecular function:flavin adenine dinucleotide binding

Features [?]

From: PS51331
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="ThyX #"
MOTIF 80 90 /note="ThyX motif #" R-H-R-x(7,8)-S

Additional information [?]

Size range 183-266 amino acids
Related rules None
Fusion None
Repeats 1-2
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.



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UniProtKB rule member sequences [?]