We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU00661
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00661
General rule information
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| Accession | PRU00661 |
| Dates | 14-APR-2009 (Created)
03-SEP-2024 (Last updated, Version 16) |
| Data class | Domain; |
| Predictors |
PROSITE; PS51331; THYX |
Name | Flavin-dependent thymidylate synthase (thyX) domain |
| Function | The thyx domain contains FAD and methylates dUMP at position 5 of the pyrimidine ring. |
| Scope(s) |
Bacteria Archaea Viruses Eukaryota Dictyostelium |
| Example(s) | O86840; |
Propagated annotation
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Identifier, protein and gene names
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| case not <Triggered> | |
| Protein name | AltName: Full=Probable thymidylate synthase; Short=TS; Short=TSase; EC=2.1.1.148; |
| end case | |
Comments
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| FUNCTION | Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant. |
| CATALYTIC ACTIVITY | Reaction=dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+); Xref=Rhea:RHEA:29043, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422; EC=2.1.1.148; |
| COFACTOR | Name=FAD; Xref=ChEBI:CHEBI:57692; synthase) domain |
Keywords
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Gene Ontology
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| GO:0008168; Molecular function:methyltransferase activity |
| GO:0016740; Molecular function:transferase activity |
| GO:0050797; Molecular function:thymidylate synthase (FAD) activity |
| GO:0006231; Biological process:dTMP biosynthetic process |
| GO:0050660; Molecular function:flavin adenine dinucleotide binding |
Features
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| From: PS51331 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="ThyX #" | |||||||||
| MOTIF | 80 | 90 | /note="ThyX motif #" | R-H-R-x(7,8)-S | ||||||||
Additional information
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| Size range | 183-266 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1-2 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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