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annotation rule: PRU00678

General rule information [?]

Accession PRU00678
Dates 18-DEC-2007 (Created)
19-NOV-2022 (Last updated, Version 10)
Data class Domain
Predictors PROSITE; PS51346; PROKAR_ZN_DEPEND_PLPC_2
Name Prokaryotic zinc-dependent phospholipase C domain
Function Phospholipase C (EC 3.1.4.3) has a ~245 amino-acids domain with the active site (PLC) in a cleft which is also occupied by 3 zinc ions. There are nine conserved residues known to be involved in binding the zinc ions: 5 His, 2 Asp, 1 Glu and 1 Trp.

Propagated annotation [?]


Description [?]

case <FTGroup:1> and <FTGroup:2> and <FTGroup:3>
+ AltName: Full=phospholipase C; EC 3.1.4.3; Short=PLC;

Comments [?]

Catalytic activity RHEA:10604: a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycerol + H(+) + phosphocholine
EC 3.1.4.3
Cofactor Zn(2+)
Note: Binds 3 Zn(2+) ions per subunit.
Subcellular location Secreted.
end case

Similarity Belongs to the bacterial zinc-metallophospholipase C family.

Cross-references [?]

case <FTGroup:1> and <FTGroup:2> and <FTGroup:3>
PROSITE PS00384; PROKAR_ZN_DEPEND_PLPC_1; 1;
end case


Gene Ontology [?]

case <FTGroup:1> or <FTGroup:2> or <FTGroup:3>
GO:0016787; Molecular function: hydrolase activity.
GO:0046872; Molecular function: metal ion binding.
GO:0008270; Molecular function: zinc ion binding.

Keywords [?]

Hydrolase
Metal-binding
Zinc
end case


Features [?]

From: PS51346
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     3     to       Zn-dependent PLC #        
BINDING     4     4       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1     W   1
BINDING     14     14       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1     H   1
BINDING     59     59       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#3     D   3
BINDING     71     71       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#3     H   3
BINDING     129     129       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#3     H   3
BINDING     133     133       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1     D   1
BINDING     133     133       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#3     D   3
BINDING     139     139       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#2     H   2
BINDING     151     151       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#2     H   2
BINDING     155     155       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#2     E   2

Additional information [?]

Size range 230-260 amino acids
Related rules None
Repeats 1
Topology Undefined
Example Q46150 (PHLC_CLONO)
Scope
Bacteria

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.


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UniProtKB rule member sequences [?]