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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00678


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PURL: https://purl.expasy.org/prosite/rule/PRU00678
General rule information [?]

Accession PRU00678
Dates 18-DEC-2007 (Created)
03-SEP-2024 (Last updated, Version 12)
Data class Domain;
Predictors PROSITE; PS51346; PROKAR_ZN_DEPEND_PLPC_2
Name Prokaryotic zinc-dependent phospholipase C domain
Function Phospholipase C (EC 3.1.4.3) has a ~245 amino-acids domain with the active site (PLC) in a cleft which is also occupied by 3 zinc ions. There are nine conserved residues known to be involved in binding the zinc ions: 5 His, 2 Asp, 1 Glu and 1 Trp.
Scope(s) Bacteria
Example(s) Q46150;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1> and <FTGroup:2> and <FTGroup:3>
Protein name + AltName: Full=phospholipase C;
                 EC=3.1.4.3;
                 Short=PLC;

Comments [?]

CATALYTIC ACTIVITY Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = phosphocholine + a 1,2-diacyl-sn-glycerol + H(+); Xref=Rhea:RHEA:10604, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:57643, ChEBI:CHEBI:295975; EC=3.1.4.3;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 3 Zn(2+) ions per subunit.;
SUBCELLULAR LOCATIONSecreted.
end case
SIMILARITYBelongs to the bacterial zinc-metallophospholipase C family.

Keywords [?]

Hydrolase
Metal-binding
Zinc
end case

Gene Ontology [?]

case <FTGroup:1> or <FTGroup:2> or <FTGroup:3>
GO:0016787; Molecular function:hydrolase activity
GO:0046872; Molecular function:metal ion binding
GO:0008270; Molecular function:zinc ion binding

Cross-references [?]

PROSITE PS00384; PROKAR_ZN_DEPEND_PLPC_1; 1;

Features [?]

From: PS51346
Key From To Description Tag Condition FTGroup
DOMAIN 3 to /note="Zn-dependent PLC #"
BINDING 4 4 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 W 1
BINDING 14 14 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 H 1
BINDING 59 59 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#3"		 D 3
BINDING 71 71 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#3"		 H 3
BINDING 129 129 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#3"		 H 3
BINDING 133 133 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 D 1
BINDING 133 133 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#3"		 D 3
BINDING 139 139 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"		 H 2
BINDING 151 151 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"		 H 2
BINDING 155 155 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"		 E 2

Additional information [?]

Size range 230-260 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]