ProRule PRU00678
General rule information
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Accession | PRU00678 |
Dates | 18-DEC-2007 (Created)
19-NOV-2022 (Last updated, Version 10) |
Data class | Domain; |
Predictors |
PROSITE; PS51346; PROKAR_ZN_DEPEND_PLPC_2 |
Name | Prokaryotic zinc-dependent phospholipase C domain |
Function | Phospholipase C (EC 3.1.4.3) has a ~245 amino-acids domain with the active site (PLC) in a cleft which is also occupied by 3 zinc ions. There are nine conserved residues known to be involved in binding the zinc ions: 5 His, 2 Asp, 1 Glu and 1 Trp. |
Scope(s) |
Bacteria |
Example(s) | Q46150 (PHLC_CLONO); |
Propagated annotation
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Identifier, protein and gene names
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case <FTGroup:1> and <FTGroup:2> and <FTGroup:3> | |
Protein name | + AltName: Full=phospholipase C; EC=3.1.4.3; Short=PLC; |
Comments
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CATALYTIC ACTIVITY | Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl- sn-glycerol + H(+) + phosphocholine; Xref=Rhea:RHEA:10604, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:57643, ChEBI:CHEBI:295975; EC=3.1.4.3; |
COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 3 Zn(2+) ions per subunit.; |
SUBCELLULAR LOCATION | Secreted. |
end case | |
SIMILARITY | Belongs to the bacterial zinc-metallophospholipase C family. |
Keywords
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Hydrolase | |
Metal-binding | |
Zinc | |
end case |
Gene Ontology
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case <FTGroup:1> or <FTGroup:2> or <FTGroup:3> | |
GO:0016787; Molecular function:hydrolase activity | |
GO:0046872; Molecular function:metal ion binding | |
GO:0008270; Molecular function:zinc ion binding |
Cross-references
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PROSITE | PS00384; PROKAR_ZN_DEPEND_PLPC_1; 1; |
Features
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From: PS51346 | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
DOMAIN | 3 | to | /note="Zn-dependent PLC #" | |||||||||
BINDING | 4 | 4 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1" |
W | 1 | |||||||
BINDING | 14 | 14 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1" |
H | 1 | |||||||
BINDING | 59 | 59 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#3" |
D | 3 | |||||||
BINDING | 71 | 71 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#3" |
H | 3 | |||||||
BINDING | 129 | 129 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#3" |
H | 3 | |||||||
BINDING | 133 | 133 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1" |
D | 1 | |||||||
BINDING | 133 | 133 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#3" |
D | 3 | |||||||
BINDING | 139 | 139 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#2" |
H | 2 | |||||||
BINDING | 151 | 151 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#2" |
H | 2 | |||||||
BINDING | 155 | 155 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#2" |
E | 2 |
Additional information
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Size range | 230-260 amino acids |
Related rules |
None |
Fusion | None |
Repeats | 1 |
Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Bacteria [10] All [ 10 ]
- Retrieve set of proteins with 3D structure for this domain