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ProRule PRU00678


General rule information [?]

Accession PRU00678
Dates 18-DEC-2007 (Created)
19-NOV-2022 (Last updated, Version 10)
Data class Domain;
Predictors PROSITE; PS51346; PROKAR_ZN_DEPEND_PLPC_2
Name Prokaryotic zinc-dependent phospholipase C domain
Function Phospholipase C (EC 3.1.4.3) has a ~245 amino-acids domain with the active site (PLC) in a cleft which is also occupied by 3 zinc ions. There are nine conserved residues known to be involved in binding the zinc ions: 5 His, 2 Asp, 1 Glu and 1 Trp.
Scope(s) Bacteria
Example(s) Q46150 (PHLC_CLONO);

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1> and <FTGroup:2> and <FTGroup:3>
Protein name + AltName: Full=phospholipase C;
                 EC=3.1.4.3;
                 Short=PLC;

Comments [?]

CATALYTIC ACTIVITY Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl- sn-glycerol + H(+) + phosphocholine; Xref=Rhea:RHEA:10604, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:57643, ChEBI:CHEBI:295975; EC=3.1.4.3;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 3 Zn(2+) ions per subunit.;
SUBCELLULAR LOCATIONSecreted.
end case
SIMILARITYBelongs to the bacterial zinc-metallophospholipase C family.

Keywords [?]


Gene Ontology [?]

case <FTGroup:1> or <FTGroup:2> or <FTGroup:3>
GO:0016787; Molecular function:hydrolase activity
GO:0046872; Molecular function:metal ion binding
GO:0008270; Molecular function:zinc ion binding

Cross-references [?]

PROSITE PS00384; PROKAR_ZN_DEPEND_PLPC_1; 1;

Features [?]

From: PS51346
Key From To Description Tag Condition FTGroup
DOMAIN 3 to /note="Zn-dependent PLC #"
BINDING 4 4 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"
W 1
BINDING 14 14 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"
H 1
BINDING 59 59 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#3"
D 3
BINDING 71 71 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#3"
H 3
BINDING 129 129 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#3"
H 3
BINDING 133 133 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"
D 1
BINDING 133 133 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#3"
D 3
BINDING 139 139 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"
H 2
BINDING 151 151 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"
H 2
BINDING 155 155 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"
E 2

Additional information [?]

Size range 230-260 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

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UniProtKB rule member sequences [?]