PRU00870

General rule information [?]

Accession	PRU00870
Dates	9-AUG-2011 (Created) 21-NOV-2019 (Last updated, Version 6)

Data class

Domain

Predictors

PROSITE; PS51537; NV_3CL_PRO

Name	Norovirus 3C-like protease (NV 3CLpro) domain

Function

NV 3CLpros belong to the chymotrypsin-like protease family, in that they appear to have chymotrypsin-like folds. Whether the 3CLpro domain has a catalytic dyad of composed of histidine and cysteine or tryad of histidine, glutamate and cysteine remains controversial.

Propagated annotation [?]

Description [?]

case <FTGroup:1>

+ Contains: RecName: Full=3C-like protease; Short=3CLpro; EC 3.4.22.66;

Comments [?]

Function	3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.
Catalytic activity	Reaction=Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-\|-Xaa and the P1' position is occupied by Gly-\|-Yaa.; EC=3.4.22.66;
Ptm	Specific enzymatic cleavages in vivo yield mature proteins. 3CLpro is first autocatalytically cleaved, then processes the whole polyprotein.

end case

Gene Ontology [?]

case <FTGroup:1>

GO:0008234; Molecular function: cysteine-type peptidase activity.
GO:0016787; Molecular function: hydrolase activity.
GO:0008233; Molecular function: peptidase activity.

Keywords [?]

Hydrolase
Protease
Thiol protease

end case

Features [?]

From: PS51537

Key From To Description Tag Condition FTGroup

DOMAIN from to Peptidase C37 #

ACT_SITE 30 30 For 3CLpro activity H 1

ACT_SITE 54 54 For 3CLpro activity E 1

ACT_SITE 139 139 For 3CLpro activity C 1

Additional information [?]

Size range	171-191 amino acids
Related rules	None
Repeats	1
Topology	Undefined
Example	P54634 (POLG_LORDV)
Scope	Viruses; Norovirus
Comments	None

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

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UniProtKB rule member sequences [?]

UniProtKB/Swiss-Prot sets

Viruses [3]
Retrieve set of proteins with 3D structure for this domain