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ProRule PRU00870


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PURL: https://purl.expasy.org/prosite/rule/PRU00870
General rule information [?]

Accession PRU00870
Dates 09-AUG-2011 (Created)
21-NOV-2019 (Last updated, Version )
Data class Domain;
Predictors PROSITE; PS51537; NV_3CL_PRO
Name Norovirus 3C-like protease (NV 3CLpro) domain
Function NV 3CLpros belong to the chymotrypsin-like protease family, in that they appear to have chymotrypsin-like folds. Whether the 3CLpro domain has a catalytic dyad of composed of histidine and cysteine or tryad of histidine, glutamate and cysteine remains controversial.
Scope(s) Viruses
Norovirus
Example(s) P54634;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + Contains:
RecName: Full=3C-like protease;
                 Short=3CLpro;
                 EC=3.4.22.66;

Comments [?]

FUNCTION3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.
CATALYTIC ACTIVITY Reaction=Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.; EC=3.4.22.66;
PTMSpecific enzymatic cleavages in vivo yield mature proteins. 3CLpro is first autocatalytically cleaved, then processes the whole polyprotein.
end case

Keywords [?]

Hydrolase
Protease
Thiol protease
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0008234; Molecular function:cysteine-type peptidase activity
GO:0016787; Molecular function:hydrolase activity
GO:0008233; Molecular function:peptidase activity

Features [?]

From: PS51537
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Peptidase C37 #"
ACT_SITE 30 30 /note="For 3CLpro activity" H 1
ACT_SITE 54 54 /note="For 3CLpro activity" E 1
ACT_SITE 139 139 /note="For 3CLpro activity" C 1

Additional information [?]

Size range 171-191 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]