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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00870


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00870
General rule information [?]

Accession PRU00870
Dates 09-AUG-2011 (Created)
21-NOV-2019 (Last updated, Version 7)
Data class Domain;
Predictors PROSITE; PS51537; NV_3CL_PRO
Name Norovirus 3C-like protease (NV 3CLpro) domain
Function NV 3CLpros belong to the chymotrypsin-like protease family, in that they appear to have chymotrypsin-like folds. Whether the 3CLpro domain has a catalytic dyad of composed of histidine and cysteine or tryad of histidine, glutamate and cysteine remains controversial.
Scope(s) Viruses
Norovirus
Example(s) P54634;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + Contains:
RecName: Full=3C-like protease;
                 Short=3CLpro;
                 EC=3.4.22.66;

Comments [?]

FUNCTION3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.
CATALYTIC ACTIVITY Reaction=Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.; EC=3.4.22.66;
PTMSpecific enzymatic cleavages in vivo yield mature proteins. 3CLpro is first autocatalytically cleaved, then processes the whole polyprotein.
end case

Keywords [?]

Hydrolase
Protease
Thiol protease
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0008234; Molecular function:cysteine-type peptidase activity
GO:0016787; Molecular function:hydrolase activity
GO:0008233; Molecular function:peptidase activity

Features [?]

From: PS51537
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Peptidase C37 #"
ACT_SITE 30 30 /note="For 3CLpro activity" H 1
ACT_SITE 54 54 /note="For 3CLpro activity" E 1
ACT_SITE 139 139 /note="For 3CLpro activity" C 1

Additional information [?]

Size range 171-191 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]