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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01085


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01085
General rule information [?]

Accession PRU01085
Dates 11-MAR-2015 (Created)
22-NOV-2019 (Last updated, Version 4)
Data class Domain;
Predictors PROSITE; PS51749; HNH_CAS9
Name Cas9-type HNH domain
Function All known Cas9 enzymes contain an HNH domain that cleaves the DNA strand complementary to the guide RNA sequence (target strand).
Scope(s) Bacteria
Example(s) Q03JI6;

Propagated annotation [?]

Keywords [?]

DNA-binding
Endonuclease
Hydrolase
Nuclease

Gene Ontology [?]

GO:0003677; Molecular function:DNA binding
GO:0003723; Molecular function:RNA binding
GO:0004519; Molecular function:endonuclease activity

Features [?]

From: PS51749
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="HNH Cas9-type #"

Additional information [?]

Size range 139-182 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]