PROSITE documentation PDOC00004
cAMP- and cGMP-dependent protein kinase phosphorylation site


There has been a number of studies relative to the specificity of cAMP- and cGMP-dependent protein kinases [1,2,3]. Both types of kinases appear to share a preference for the phosphorylation of serine or threonine residues found close to at least two consecutive N-terminal basic residues. It is important to note that there are quite a number of exceptions to this rule.

Last update:

June 1988 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

CAMP_PHOSPHO_SITE, PS00004; cAMP- and cGMP-dependent protein kinase phosphorylation site  (PATTERN with a high probability of occurrence!)


1AuthorsFremisco J.R. Glass D.B. Krebs E.G.
SourceJ. Biol. Chem. 255:4240-4245(1980).

2AuthorsGlass D.B. Smith S.B.
TitlePhosphorylation by cyclic GMP-dependent protein kinase of a synthetic peptide corresponding to the autophosphorylation site in the enzyme.
SourceJ. Biol. Chem. 258:14797-14803(1983).
PubMed ID6317673

3AuthorsGlass D.B. el-Maghrabi M.R. Pilkis S.J.
TitleSynthetic peptides corresponding to the site phosphorylated in 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as substrates of cyclic nucleotide-dependent protein kinases.
SourceJ. Biol. Chem. 261:2987-2993(1986).
PubMed ID3005275

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