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PROSITE documentation PDOC00131recA family signature and profiles
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00131
The bacterial recA protein [1,2,3] is essential for homologous recombination and recombinational repair of DNA damage. RecA has many activities: it filaments, it binds to single- and double-stranded DNA, it binds and hydrolyzes ATP, it is also a recombinase and, finally, it interacts with lexA causing its activation and leading to its autocatalytic cleavage.
RecA is a protein of about 350 amino-acid residues. Its sequence is very well conserved [3,4,5] among eubacterial species. It is also found in the chloroplast of plants [6].
The recA protein is closely related to:
- Eukaryotic RAD51 protein. Promotes homologous pairing and strand exchange on chromatin.
- Eukaryotic DMC1 protein. Participates in meiotic recombination.
- Prokaryotic radA protein. Involved in DNA repair and in homologous recombination.
- Bacteriophage uvsX gene product. Important in genetic recombination, DNA repair, and replication.
As a signature pattern specific for the bacterial and chloroplastic recA protein, we selected the best conserved region, a nonapeptide located in the middle of the sequence and which is part of the monomer-monomer interface in a recA filament.
We also developed two profiles. The first one covers the ATP binding domain in the N-terminal part of the recA protein. The second one span the whole monomer-monomer interface. These two profiles also pick up the recA-like proteins.
Eisen J.A.; jeisen@leland.stanford.eduExpert(s) to contact by email:
Roca A.I.;
Last update:December 2012 / Profile revised.
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PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Smith K.C. Wang T.-C. |
| Title | recA-dependent DNA repair processes. | |
| Source | BioEssays 10:12-16(1989). | |
| PubMed ID | 2653307 |
| 2 | Authors | Lloyd A.T. Sharp P.M. |
| Title | Evolution of the recA gene and the molecular phylogeny of bacteria. | |
| Source | J. Mol. Evol. 37:399-407(1993). | |
| PubMed ID | 8308907 |
| 3 | Authors | Roca A.I. Cox M.M. |
| Source | Prog. Nucleic Acids Res. Mol. Biol. 56:129-223(1997). |
| 4 | Authors | Karlin S. Weinstock G.M. Brendel V. |
| Title | Bacterial classifications derived from recA protein sequence comparisons. | |
| Source | J. Bacteriol. 177:6881-6893(1995). | |
| PubMed ID | 7592482 |
| 5 | Authors | Eisen J.A. |
| Title | The RecA protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of RecAs and 16S rRNAs from the same species. | |
| Source | J. Mol. Evol. 41:1105-1123(1995). | |
| PubMed ID | 8587109 |
| 6 | Authors | Cerutti H.D. Osman M. Grandoni P. Jagendorf A.T. |
| Title | A homolog of Escherichia coli RecA protein in plastids of higher plants. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 89:8068-8072(1992). | |
| PubMed ID | 1518831 |
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