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Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00166Thiamine pyrophosphate enzymes signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00166
Description
A number of enzymes require thiamine pyrophosphate (TPP) (vitamin B1) as a cofactor. It has been shown [1] that some of these enzymes are structurally related. These related TPP enzymes are:
- Pyruvate oxidase (POX) (EC 1.2.3.3) Reaction catalyzed: pyruvate + orthophosphate + O(2) + H(2)O = acetyl phosphate + CO(2) + H(2)O(2).
- Pyruvate decarboxylase (PDC) (EC 4.1.1.1) Reaction catalyzed: pyruvate = acetaldehyde + CO(2).
- Indolepyruvate decarboxylase (EC 4.1.1.74) [2] Reaction catalyzed: indole-3-pyruvate = indole-3-acetaldehyde + CO(2).
- Acetolactate synthase (ALS) (EC 2.2.1.6) Reaction catalyzed: 2 pyruvate = acetolactate + CO(2).
- Benzoylformate decarboxylase (BFD) (EC 4.1.1.7) [3] Reaction catalyzed: benzoylformate = benzaldehyde + CO(2).
As a signature pattern for these enzymes we have selected a conserved region which is located in their C-terminal section.
Note:Other TPP enzymes such as the E1 component of pyruvate dehydrogenase complex, 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase, and transketolase do not seem to be related to the above enzymes.
Last update:November 1995 / Pattern and text revised.
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Technical section
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References
| 1 | Authors | Green J.B.A. |
| Title | Pyruvate decarboxylase is like acetolactate synthase (ILV2) and not like the pyruvate dehydrogenase E1 subunit. | |
| Source | FEBS Lett. 246:1-5(1989). | |
| PubMed ID | 2651151 |
| 2 | Authors | Koga J. Adachi T. Hidaka H. |
| Title | Molecular cloning of the gene for indolepyruvate decarboxylase from Enterobacter cloacae. | |
| Source | Mol. Gen. Genet. 226:10-16(1991). | |
| PubMed ID | 2034209 |
| 3 | Authors | Tsou A.Y. Ransom S.C. Gerlt J.A. Buechter D.D. Babbitt P.C. Kenyon G.L. |
| Title | Mandelate pathway of Pseudomonas putida: sequence relationships involving mandelate racemase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase and expression of benzoylformate decarboxylase in Escherichia coli. | |
| Source | Biochemistry 29:9856-9862(1990). | |
| PubMed ID | 2271624 |
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