PROSITE documentation PDOC00303
Transcription factor TFIID repeat signature


Transcription factor TFIID (or TATA-binding protein, TBP) [1,2] is a general factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. TFIID binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.

There is a remarkable degree of sequence conservation of a C-terminal domain of about 180 residues in TFIID from various eukaryotic sources. This region is necessary and sufficient for TATA box binding. The most significant structural feature of this domain is the presence of two conserved repeats of a 77 amino-acid region. The intramolecular symmetry generates a saddle-shaped structure that sits astride the DNA [3].

Drosophila TRF (TBP-related factor) [4] is a sequence-specific transcription factor that also binds to the TATA box and is highly similar to TFIID.

Archaebacteria also possess a TBP homolog [5].

We have derived a signature pattern that spans the last 50 residues of the repeated region.

Last update:

December 2004 / Pattern and text revised.


Technical section

PROSITE method (with tools and information) covered by this documentation:

TFIID, PS00351; Transcription factor TFIID repeat signature  (PATTERN)


1AuthorsHoffmann A. Sinn E. Yamamoto T. Wang J. Roy A. Horikoshi M. Roeder R.G.
SourceNature 346:387-390(1990).

2AuthorsGash A. Hoffmann A. Horikoshi M. Roeder R.G. Chua N.-H.
SourceNature 346:390-394(1990).

3AuthorsNikolov D.B. Hu S.-H. Lin J. Gasch A. Hoffmann A. Horikoshi M. Chua N.-H. Roeder R.G. Burley S.K.
TitleCrystal structure of TFIID TATA-box binding protein.
SourceNature 360:40-46(1992).
PubMed ID1436073

4AuthorsCrowley T.E. Hoey T. Liu J.-K. Jan Y.N. Jan L.Y. Tjian R.
TitleA new factor related to TATA-binding protein has highly restricted expression patterns in Drosophila.
SourceNature 361:557-561(1993).
PubMed ID8429912

5AuthorsMarsh T.L. Reich C.I. Whitelock R.B. Olsen G.J.
TitleTranscription factor IID in the Archaea: sequences in the Thermococcus celer genome would encode a product closely related to the TATA-binding protein of eukaryotes.
SourceProc. Natl. Acad. Sci. U.S.A. 91:4180-4184(1994).
PubMed ID8183889

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