PROSITE documentation PDOC00317Ribonucleotide reductase small subunit signature
Ribonucleotide reductase (EC 1.17.4.1) [1,2] catalyzes the reductive synthesis of deoxyribonucleotides from their corresponding ribonucleotides. It provides the precursors necessary for DNA synthesis. Ribonucleotide reductase is an oligomeric enzyme composed of a large subunit (700 to 1000 residues) and a small subunit (300 to 400 residues).
The small chain binds two iron atoms [3] (three Glu, one Asp, and two His are involved in metal binding) and contains an active site tyrosine radical. The regions of the sequence that contain the metal-binding residues and the active site tyrosine are conserved in ribonucleotide reductase small chain from prokaryotes, eukaryotes and viruses.
We have selected one of these regions as a signature pattern. It contains the active site residue as well as a glutamate and a histidine involved in the binding of iron.
Last update:December 2001 / Pattern and text revised.
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| 1 | Authors | Nillson O. Lundqvist T. Hahne S. Sjoberg B.-M. |
| Source | Biochem. Soc. Trans. 16:91-94(1988). |
| 2 | Authors | Reichard P. |
| Title | From RNA to DNA, why so many ribonucleotide reductases? | |
| Source | Science 260:1773-1777(1993). | |
| PubMed ID | 8511586 |
| 3 | Authors | Nordlund P. Sjoberg B.-M. Eklund H. |
| Title | Three-dimensional structure of the free radical protein of ribonucleotide reductase. | |
| Source | Nature 345:593-598(1990). | |
| PubMed ID | 2190093 | |
| DOI | 10.1038/345593a0 |
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