PROSITE documentation PDOC00400Nickel-dependent hydrogenases large subunit signatures
Hydrogenases are enzymes that catalyze the reversible activation of hydrogen and which occur widely in prokaryotes as well as in some eukaryotes. There are various types of hydrogenases, but all of them seem to contain at least one iron-sulfur cluster. They can be broadly divided into two groups: hydrogenases containing nickel and, in some cases, also selenium (the [NiFe] and [NiFeSe] hydrogenases) and those lacking nickel (the [Fe] hydrogenases).
The [NiFe] and [NiFeSe] hydrogenases are heterodimer that consist of a small subunit that contains a signal peptide and a large subunit. All the known large subunits seem to be evolutionary related [1]; they contain two Cys-x-x-Cys motifs; one at their N-terminal end; the other at their C-terminal end. These four cysteines are involved in the binding of nickel [2]. In the [NiFeSe] hydrogenases the first cysteine of the C-terminal motif is a selenocysteine which has experimentally been shown to be a nickel ligand [3]. We have developed two patterns which are centered on the Cys-x-x-Cys motifs.
Alcaligenes eutrophus possess a NAD-reducing cytoplasmic hydrogenase (hoxS) [4]; this enzyme is composed of four subunits. Two of these subunits (β and delta) are responsible for the hydrogenase reaction and are evolutionary related to the large and small subunits of membrane-bound hydrogenases.
The α subunit of coenzyme F420 hydrogenase (EC 1.12.98.1) (FRH) from archaebacterial methanogens also belongs to this family.
Last update:January 2008 / Pattern updated.
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PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Menon N.K. Robbins J. Peck H.D. Jr. Chatelus C.Y. Choi E.-S. Przybyla A.E. |
| Title | Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames. | |
| Source | J. Bacteriol. 172:1969-1977(1990). | |
| PubMed ID | 2180913 |
| 2 | Authors | Volbeda A. Charon M.-H. Piras C. Hatchikian E.C. Frey M. Fontecilla-Camps J.C. |
| Title | Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas. | |
| Source | Nature 373:580-587(1995). | |
| PubMed ID | 7854413 | |
| DOI | 10.1038/373580a0; |
| 3 | Authors | Eidsness M.K. Scott R.A. Prickrill B. der Vartaninan D.V. LeGall J. Moura I. Moura J.J.G. Peck H.D. Jr. |
| Title | vidence for selenocysteine coordination to the active site nickel in the [NiFeSe]hydrogenases from Desulfovibrio baculatus. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 86:147-151(1989). | |
| PubMed ID | 2521386 |
| 4 | Authors | Tran-Betcke A. Warnecke U. Boecker C. Zaborosch C. Friedrich B. |
| Title | Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16. | |
| Source | J. Bacteriol. 172:2920-2929(1990). | |
| PubMed ID | 2188945 |
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