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PROSITE documentation PDOC00404Gamma-glutamyltranspeptidase signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00404
γ-glutamyltranspeptidase (EC 2.3.2.2) (GGT) [1] catalyzes the transfer of the γ-glutamyl moiety of glutathione to an acceptor that may be an amino acid, a peptide or water (forming glutamate). GGT plays a key role in the γ-glutamyl cycle, a pathway for the synthesis and degradation of glutathione. In prokaryotes and eukaryotes, it is an enzyme that consists of two polypeptide chains, a heavy and a light subunit, processed from a single chain precursor. The active site of GGT is known to be located in the light subunit.
The sequences of mammalian and bacterial GGT show a number of regions of high similarity [2]. Pseudomonas cephalosporin acylases (EC 3.5.1.-) that convert 7-β-(4-carboxybutanamido)-cephalosporanic acid (GL-7ACA) into 7-aminocephalosporanic acid (7ACA) and glutaric acid are evolutionary related to GGT and also show some GGT activity [3]. Like GGT, these GL-7ACA acylases, are also composed of two subunits.
One of the conserved regions correspond to the N-terminal extremity of the mature light chains of these enzymes. We have used this region as a signature pattern.
Last update:November 1997 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Tate S.S. Meister A. |
| Title | Gamma-glutamyl transpeptidase from kidney. | |
| Source | Methods Enzymol. 113:400-419(1985). | |
| PubMed ID | 2868390 |
| 2 | Authors | Suzuki H. Kumagai H. Echigo T. Tochikura T. |
| Title | DNA sequence of the Escherichia coli K-12 gamma-glutamyltranspeptidase gene, ggt. | |
| Source | J. Bacteriol. 171:5169-5172(1989). | |
| PubMed ID | 2570061 |
| 3 | Authors | Ishiye M. Niwa M. |
| Title | Nucleotide sequence and expression in Escherichia coli of the cephalosporin acylase gene of a Pseudomonas strain. | |
| Source | Biochim. Biophys. Acta 1132:233-239(1992). | |
| PubMed ID | 1358202 |
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