PROSITE documentation PDOC00470

Methylmalonyl-CoA mutase signature





Description

Methylmalonyl-CoA mutase (EC 5.4.99.2) (MCM) [1] is an adenosylcobalamin (vitamin B12) dependent enzyme that catalyzes the isomerization between methylmalonyl-CoA and succinyl-CoA. MCM is involved in various catabolic or biosynthetic pathways; for example in man it is involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle; while in some bacteria it is involved in the synthesis of propionate from tricarboxylic acid-cycle intermediates.

Deficiency of MCM in man causes an often fatal disorder of organic acid metabolism termed methylmalonic acidemia.

In eukaryotes MCM is located in the mitochondrial matrix and is a homodimer of a polypeptide chain of about 710 amino acids. In bacteria MCM is a dimer of two non-identical, yet structurally related chains.

The sequences of eukaryotic and prokaryotic MCM are rather well conserved. This family also includes an Escherichia coli protein (gene sbm) whose function is not yet known.

As a signature pattern we selected the best conserved region which is located in the central part of these sequences.

Note:

A small degree of similarity is said [2] to exist between MCM and the large subunit of the adenosylcobalamin-dependent enzyme ethanolamine ammonia- lyase, but this similarity is so weak that these two type of enzymes can not be detected by a single pattern.

Last update:

December 2001 / Pattern and text revised.

Technical section

PROSITE method (with tools and information) covered by this documentation:

METMALONYL_COA_MUTASE, PS00544; Methylmalonyl-CoA mutase signature  (PATTERN)


References

1AuthorsLedley F.D.
TitlePerspectives on methylmalonic acidemia resulting from molecular cloning of methylmalonyl CoA mutase.
SourceBioEssays 12:335-340(1990).
PubMed ID1975493

2AuthorsFaust L.R.P., Connor J.A., Roof D.M., Hoch J.A., Babior B.M.
TitleCloning, sequencing, and expression of the genes encoding the adenosylcobalamin-dependent ethanolamine ammonia-lyase of Salmonella typhimurium.
SourceJ. Biol. Chem. 265:12462-12466(1990).
PubMed ID2197274



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