|PROSITE documentation PDOC00479|
The TEA domain is a DNA-binding region of ~80 amino acids that has been named after the two proteins that originally defined the domain: TEF-1 and AbaA. It is also referred to as the ATTS domain because of the 4 proteins first found to harbor this motif (AbaA and TEC1 in yeast, TEF1/TEAD1 in vertebrates and Scalloped in fly). The TEA domain is located toward the amino terminus of eukaryotic transcription factors of the TEA/ATTS family. The consensus DNA sequence bound by an isolated TEA domain is N-[ATG]-G-[ATC]-A-T-N-T; where, N=A, T, C. or G [1,2,3,4].
The TEA domain is composed of a three-helix bundle with a homeodomain fold (see <PDB:2HZD>). It has a folded globular structure made of three α-helices, H1, H2, and H3. H1 and H2 are nearly anti-parallel and pack on either side of the H3, which is the DNA-recognition helix of the TEA domain. Two conserved serines are found on the DNA-binding surface. Phosphorylation of one or both of these could interfere with DNA-binding activity, by introducing electrostatic repulsion and/or steric hindrance, and help regulate TEA/ATTS transcription factor activity [3,4].
Some of the proteins that contain a TEA domain are listed below:
We developed both a pattern and a profile. The pattern covers helices H2 and H3, whereas the profile covers the whole domain.Last update:
January 2017 / Profile and text revised.
PROSITE methods (with tools and information) covered by this documentation:
|Title||The TEA domain: a novel, highly conserved DNA-binding motif.|
|2||Authors||Hwang J.J. Chambon P. Davidson I.|
|Title||Characterization of the transcription activation function and the DNA binding domain of transcriptional enhancer factor-1.|
|Source||EMBO J. 12:2337-2348(1993).|
|3||Authors||Anbanandam A. Albarado D.C. Nguyen C.T. Halder G. Gao X. Veeraraghavan S.|
|Title||Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain.|
|Source||Proc. Natl. Acad. Sci. U.S.A. 103:17225-17230(2006).|
|4||Authors||Landin-Malt A. Benhaddou A. Zider A. Flagiello D.|
|Title||An evolutionary, structural and functional overview of the mammalian TEAD1 and TEAD2 transcription factors.|
|5||Authors||Jacquemin P. Hwang J.-J. Martial J.A. Dolle P. Davidson I.|
|Title||A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain.|
|Source||J. Biol. Chem. 271:21775-21785(1996).|
|6||Authors||Campbell S. Inamdar M. Rodrigues V. Raghavan V. Palazzolo M. Chovnick A.|
|Title||The scalloped gene encodes a novel, evolutionarily conserved transcription factor required for sensory organ differentiation in Drosophila.|
|Source||Genes Dev. 6:367-379(1992).|