PROSITE logo
Black ribbon
We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE documentation PDOC00504
pfkB family of carbohydrate kinases signatures


View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC00504

Description

It has been shown [1,2,3] that the following carbohydrate and purine kinases are evolutionary related and can be grouped into a single family, which is known [1] as the 'pfkB family':

  • Fructokinase (EC 2.7.1.4) (gene scrK).
  • 6-phosphofructokinase isozyme 2 (EC 2.7.1.11) (phosphofructokinase-2) (gene pfkB). pfkB is a minor phosphofructokinase isozyme in Escherichia coli and is not evolutionary related to the major isozyme (gene pfkA). Plants 6-phosphofructokinase also belong to this family.
  • Ribokinase (EC 2.7.1.15) (gene rbsK).
  • Adenosine kinase (EC 2.7.1.20) (gene ADK).
  • 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45) (gene: kdgK).
  • 1-phosphofructokinase (EC 2.7.1.56) (fructose 1-phosphate kinase) (gene fruK).
  • Inosine-guanosine kinase (EC 2.7.1.73) (gene gsk).
  • Tagatose-6-phosphate kinase (EC 2.7.1.144) (phosphotagatokinase) (gene lacC).
  • Escherichia coli hypothetical protein yeiC.
  • Escherichia coli hypothetical protein yeiI.
  • Escherichia coli hypothetical protein yhfQ.
  • Escherichia coli hypothetical protein yihV.
  • Bacillus subtilis hypothetical protein yxdC.
  • Yeast hypothetical protein YJR105w.

All the above kinases are proteins of from 280 to 430 amino acid residues that share a few region of sequence similarity. We have selected two of these regions as signature patterns. The first pattern is based on a region rich in glycine which is located in the N-terminal section of these enzymes; while the second pattern is based on a conserved region in the C-terminal section.

Note:

Some bacterial fructokinases belong to the ROK family <PDOC00866>.

Last update:

April 2006 / Pattern revised.

-------------------------------------------------------------------------------


Technical section

PROSITE methods (with tools and information) covered by this documentation:

PFKB_KINASES_1, PS00583; pfkB family of carbohydrate kinases signature 1  (PATTERN)

PFKB_KINASES_2, PS00584; pfkB family of carbohydrate kinases signature 2  (PATTERN)


References

1AuthorsWu L.-F. Reizer A. Reizer J. Cai B. Tomich J.M. Saier M.H. Jr.
TitleNucleotide sequence of the Rhodobacter capsulatus fruK gene, which encodes fructose-1-phosphate kinase: evidence for a kinase superfamily including both phosphofructokinases of Escherichia coli.
SourceJ. Bacteriol. 173:3117-3127(1991).
PubMed ID1850730

2AuthorsOrchard L.M.D. Kornberg H.L.
TitleSequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus.
SourceProc. R. Soc. Lond., B, Biol. Sci. 242:87-90(1990).
PubMed ID1981619

3AuthorsBlatch G.L. Scholle R.R. Woods D.R.
TitleNucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region.
SourceGene 95:17-23(1990).
PubMed ID2174811



PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.