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	PROSITE documentation PDOC00504

pfkB family of carbohydrate kinases signatures

It has been shown [1,2,3] that the following carbohydrate and purine kinases are evolutionary related and can be grouped into a single family, which is known [1] as the 'pfkB family':

• Fructokinase (EC 2.7.1.4) (gene scrK).
• 6-phosphofructokinase isozyme 2 (EC 2.7.1.11) (phosphofructokinase-2) (gene pfkB). pfkB is a minor phosphofructokinase isozyme in Escherichia coli and is not evolutionary related to the major isozyme (gene pfkA). Plants 6-phosphofructokinase also belong to this family.
• Ribokinase (EC 2.7.1.15) (gene rbsK).
• Adenosine kinase (EC 2.7.1.20) (gene ADK).
• 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45) (gene: kdgK).
• 1-phosphofructokinase (EC 2.7.1.56) (fructose 1-phosphate kinase) (gene fruK).
• Inosine-guanosine kinase (EC 2.7.1.73) (gene gsk).
• Tagatose-6-phosphate kinase (EC 2.7.1.144) (phosphotagatokinase) (gene lacC).
• Escherichia coli hypothetical protein yeiC.
• Escherichia coli hypothetical protein yeiI.
• Escherichia coli hypothetical protein yhfQ.
• Escherichia coli hypothetical protein yihV.
• Bacillus subtilis hypothetical protein yxdC.
• Yeast hypothetical protein YJR105w.

All the above kinases are proteins of from 280 to 430 amino acid residues that share a few region of sequence similarity. We have selected two of these regions as signature patterns. The first pattern is based on a region rich in glycine which is located in the N-terminal section of these enzymes; while the second pattern is based on a conserved region in the C-terminal section.

Note:

Some bacterial fructokinases belong to the ROK family <PDOC00866>.

Last update:

April 2006 / Pattern revised.

PROSITE methods (with tools and information) covered by this documentation:

PFKB_KINASES_1, PS00583; pfkB family of carbohydrate kinases signature 1  (PATTERN)

• Consensus pattern:
  [AG]-G-x(0,1)-[GAP]-x-N-{AGLS}-[STA]-x(2)-{A}-x-{G}-{GNKA}-[GS]-x(9)-G
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 383
  • detected by PS00583: 252 (true positives)
  • undetected by PS00583: 131 (130 false negatives and 1 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS00583:
  16 false positives.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00583
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00583
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS00583
• View ligand binding statistics of PS00583
• Matching PDB structures: 2AWD 2C49 2C4E 2F02 ... [ALL]

PFKB_KINASES_2, PS00584; pfkB family of carbohydrate kinases signature 2  (PATTERN)

• Consensus pattern:
  [DNSK]-[PSTV]-x-[SAG](2)-[GD]-D-x(3)-[SAGV]-[AG]-[LIVMFYA]-[LIVMSTAP]
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 390
  • detected by PS00584: 172 (true positives)
  • undetected by PS00584: 218 (218 false negatives and 0 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS00584:
  23 false positives.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00584
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00584
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS00584
• View ligand binding statistics of PS00584
• Matching PDB structures: 1BX4 1DGM 1GQT 1LII ... [ALL]

1	Authors	Wu L.-F., Reizer A., Reizer J., Cai B., Tomich J.M., Saier M.H. Jr.
	Title	Nucleotide sequence of the Rhodobacter capsulatus fruK gene, which encodes fructose-1-phosphate kinase: evidence for a kinase superfamily including both phosphofructokinases of Escherichia coli.
	Source	J. Bacteriol. 173:3117-3127(1991).
	PubMed ID	1850730

2	Authors	Orchard L.M.D., Kornberg H.L.
	Title	Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus.
	Source	Proc. R. Soc. Lond., B, Biol. Sci. 242:87-90(1990).
	PubMed ID	1981619

3	Authors	Blatch G.L., Scholle R.R., Woods D.R.
	Title	Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region.
	Source	Gene 95:17-23(1990).
	PubMed ID	2174811

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