PROSITE documentation PDOC00523
Fructose-bisphosphate aldolase class-II signatures


Fructose-bisphosphate aldolase (EC [1,2] is a glycolytic enzyme that catalyzes the reversible aldol cleavage or condensation of fructose-1,6-bisphosphate into dihydroxyacetone-phosphate and glyceraldehyde 3-phosphate. There are two classes of fructose-bisphosphate aldolases with different catalytic mechanisms. Class-II aldolases [2], mainly found in prokaryotes and fungi, are homodimeric enzymes which require a divalent metal ion - generally zinc - for their activity.

This family also includes the following proteins:

  • Escherichia coli galactitol operon protein gatY which catalyzes the transformation of tagatose 1,6-bisphosphate into glycerone phosphate and D- glyceraldehyde 3-phosphate.
  • Escherichia coli N-acetyl galactosamine operon protein agaY which catalyzes the same reaction as that of gatY.

As signature patterns for this class of enzyme, we selected two conserved regions. The first pattern is located in the first half of the sequence and contains two histidine residues that have been shown [4] to be involved in binding a zinc ion. The second is located in the C-terminal section and contains clustered acidic residues and glycines.

Last update:

December 2001 / Pattern and text revised.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

ALDOLASE_CLASS_II_1, PS00602; Fructose-bisphosphate aldolase class-II signature 1  (PATTERN)

ALDOLASE_CLASS_II_2, PS00806; Fructose-bisphosphate aldolase class-II signature 2  (PATTERN)


1AuthorsPerham R.N.
TitleThe fructose-1,6-bisphosphate aldolases: same reaction, different enzymes.
SourceBiochem. Soc. Trans. 18:185-187(1990).
PubMed ID2199259

2AuthorsMarsh J.J. Lebherz H.G.
TitleFructose-bisphosphate aldolases: an evolutionary history.
SourceTrends Biochem. Sci. 17:110-113(1992).
PubMed ID1412694

3Authorsvon der Osten C.H. Barbas C.F. III Wong C.-H. Sinskey A.J.
SourceMol. Microbiol. 3:1625-1637(1989).

4AuthorsBerry A. Marshall K.E.
TitleIdentification of zinc-binding ligands in the class II fructose-1,6-bisphosphate aldolase of Escherichia coli.
SourceFEBS Lett. 318:11-16(1993).
PubMed ID8436219

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