PROSITE logo
Black ribbon
We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE documentation PDOC00538
Histidine acid phosphatases signatures


View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC00538

Description

Acid phosphatases (EC 3.1.3.2) are a heterogeneous group of proteins that hydrolyze phosphate esters, optimally at low pH. It has been shown [1] that a number of acid phosphatases, from both prokaryotes and eukaryotes, share two regions of sequence similarity, each centered around a conserved histidine residue. These two histidines seem to be involved in the enzymes' catalytic mechanism [2,3]. The first histidine is located in the N-terminal section and forms a phosphohistidine intermediate while the second is located in the C-terminal section and possibly acts as proton donor. Enzymes belonging to this family are called 'histidine acid phosphatases' and are listed below:

  • Escherichia coli pH 2.5 acid phosphatase (gene appA).
  • Escherichia coli glucose-1-phosphatase (EC 3.1.3.10) (gene agp).
  • Yeast constitutive and repressible acid phosphatases (genes PHO3 and PHO5).
  • Fission yeast acid phosphatase (gene pho1).
  • Aspergillus phytases A and B (EC 3.1.3.8) (gene phyA and phyB).
  • Mammalian lysosomal acid phosphatase.
  • Mammalian prostatic acid phosphatase.
  • Caenorhabditis elegans hypothetical proteins B0361.7, C05C10.1, C05C10.4 and F26C11.1.
Last update:

April 2006 / Pattern revised.

-------------------------------------------------------------------------------


Technical section

PROSITE methods (with tools and information) covered by this documentation:

HIS_ACID_PHOSPHAT_1, PS00616; Histidine acid phosphatases phosphohistidine signature  (PATTERN)

HIS_ACID_PHOSPHAT_2, PS00778; Histidine acid phosphatases active site signature  (PATTERN)


References

1Authorsvan Etten R.L. Davidson R. Stevis P.E. MacArthur H. Moore D.L.
SourceJ. Biol. Chem. 266:2313-2319(1991).

2AuthorsOstanin K. Harms E.H. Stevis P.E. Kuciel R. Zhou M.-M. Van Etten R.L.
TitleOverexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase.
SourceJ. Biol. Chem. 267:22830-22836(1992).
PubMed ID1429631

3AuthorsSchneider G. Lindqvist Y. Vihko P.
TitleThree-dimensional structure of rat acid phosphatase.
SourceEMBO J. 12:2609-2615(1993).
PubMed ID8334986



PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.