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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
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Amos Bairoch

PROSITE documentation PDOC00539
recF protein signatures


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PURL: https://purl.expasy.org/prosite/documentation/PDOC00539

Description

The prokaryotic protein recF [1,2] is a single-stranded DNA-binding protein which also probably binds ATP. RecF is involved in DNA metabolism; it is required for recombinational DNA repair and for induction of the SOS response.

RecF is a protein of about 350 to 370 amino acid residues; there is a conserved ATP-binding site motif 'A' (P-loop) in the N-terminal section of the protein as well as two other conserved regions, one located in the central section, and the other in the C-terminal section. We have derived signature patterns from these two regions.

Last update:

April 2006 / Pattern revised.

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Technical section

PROSITE methods (with tools and information) covered by this documentation:

RECF_1, PS00617; RecF protein signature 1  (PATTERN)

RECF_2, PS00618; RecF protein signature 2  (PATTERN)


References

1AuthorsSandler S.J. Chackerian B. Li J.T. Clark A.J.
TitleSequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop.
SourceNucleic Acids Res. 20:839-845(1992).
PubMed ID1542576

2AuthorsAlonso J.C. Fisher L.M.
TitleNucleotide sequence of the recF gene cluster from Staphylococcus aureus and complementation analysis in Bacillus subtilis recF mutants.
SourceMol. Gen. Genet. 246:680-686(1995).
PubMed ID7898435



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