Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00557Thymidine and pyrimidine-nucleoside phosphorylases signature
View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC00557
Thymidine phosphorylase (EC 2.4.2.4) catalyzes the reversible phosphorolysis of thymidine, deoxyuridine and their analogues to their respective bases and 2-deoxyribose 1-phosphate. This enzyme regulates the availability of thymidine and is therefore essential to nucleic acid metabolism.
In Escherichia coli (gene deoA), the enzyme is a dimer of identical subunits of about 48 Kd [1]. In humans it was first identified as platelet-derived endothelial cell growth factor (PD-ECGF) before being recognized [2] as thymidine phosphorylase.
Bacterial pyrimidine-nucleoside phosphorylase (EC 2.4.2.2) (gene pdp) [3] is an enzyme evolutionary and structurally related to thymidine phosphorylase.
As a signature pattern for these enzymes, we selected a well conserved region of 19 residues located in the N-terminal part of these proteins.
Last update:April 2006 / Pattern revised.
-------------------------------------------------------------------------------
PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Walter M.R. Cook W.J. Cole L.B. Short S.A. Koszalka G.W. Krenitsky T.A. Ealick S.E. |
| Title | Three-dimensional structure of thymidine phosphorylase from Escherichia coli at 2.8 A resolution. | |
| Source | J. Biol. Chem. 265:14016-14022(1990). | |
| PubMed ID | 2199449 |
| 2 | Authors | Furukawa T. Yoshimura A. Sumizawa T. Haraguchi M. Akiyama S.-I. Fukui K. Ishizawa M. Yamada Y. |
| Title | Angiogenic factor. | |
| Source | Nature 356:668-668(1992). | |
| PubMed ID | 1570012 | |
| DOI | 10.1038/356668a0 |
| 3 | Authors | Saxild H.H. Andersen L.N. Hammer K. |
| Title | Dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein. | |
| Source | J. Bacteriol. 178:424-434(1996). | |
| PubMed ID | 8550462 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.