Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00558Bacterial ribonuclease P protein component signature
View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC00558
Ribonuclease P (EC 3.1.26.5) (RNase P) [1,2,3] is a site specific endonuclease that generates mature tRNAs by cleaving-off the leader sequences at their 5'ends. In bacteria RNase P is known to be composed of two components: a large (about 400 base pairs) RNA (gene rnpB) and a small protein (119 to 133 amino acids) (gene rnpA). The RNA moiety of RNase P carries the catalytic activity; the function of the protein component is not yet clear although it may act as an electrostatic screen allowing the highly negatively charged RNA enzyme-substrate complex to fold into the catalytic conformation.
The sequence of rnpA is not highly conserved, however there is, in the central part of the protein, a conserved basic region. We have used this region as a signature pattern.
Last update:December 2004 / Pattern and text revised.
-------------------------------------------------------------------------------
PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Pace N.R. Smith D. |
| Title | Ribonuclease P: function and variation. | |
| Source | J. Biol. Chem. 265:3587-3590(1990). | |
| PubMed ID | 1689306 |
| 2 | Authors | Altman S. |
| Title | Ribonuclease P. Postscript. | |
| Source | J. Biol. Chem. 265:20053-20056(1990). | |
| PubMed ID | 1700778 |
| 3 | Authors | Brown J.W. Pace N.R. |
| Title | Ribonuclease P RNA and protein subunits from bacteria. | |
| Source | Nucleic Acids Res. 20:1451-1456(1992). | |
| PubMed ID | 1374553 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.