PROSITE documentation PDOC00558Bacterial ribonuclease P protein component signature
Ribonuclease P (EC 3.1.26.5) (RNase P) [1,2,3] is a site specific endonuclease that generates mature tRNAs by cleaving-off the leader sequences at their 5'ends. In bacteria RNase P is known to be composed of two components: a large (about 400 base pairs) RNA (gene rnpB) and a small protein (119 to 133 amino acids) (gene rnpA). The RNA moiety of RNase P carries the catalytic activity; the function of the protein component is not yet clear although it may act as an electrostatic screen allowing the highly negatively charged RNA enzyme-substrate complex to fold into the catalytic conformation.
The sequence of rnpA is not highly conserved, however there is, in the central part of the protein, a conserved basic region. We have used this region as a signature pattern.
Last update:December 2004 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Pace N.R. Smith D. |
| Title | Ribonuclease P: function and variation. | |
| Source | J. Biol. Chem. 265:3587-3590(1990). | |
| PubMed ID | 1689306 |
| 2 | Authors | Altman S. |
| Title | Ribonuclease P. Postscript. | |
| Source | J. Biol. Chem. 265:20053-20056(1990). | |
| PubMed ID | 1700778 |
| 3 | Authors | Brown J.W. Pace N.R. |
| Title | Ribonuclease P RNA and protein subunits from bacteria. | |
| Source | Nucleic Acids Res. 20:1451-1456(1992). | |
| PubMed ID | 1374553 |
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