PROSITE documentation PDOC00586
Prokaryotic-type carbonic anhydrases signatures


Carbonic anhydrases (EC (CA) are zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide.

In Escherichia coli, CA (gene cynT) is involved in recycling carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (gene cynS). By this action, it prevents the depletion of cellular bicarbonate [1]. In photosynthetic bacteria and plant chloroplast, CA is essential to inorganic carbon fixation [2].

Prokaryotic and plant chloroplast CA are structurally and evolutionary related and form a family distinct from the one which groups the many different forms of eukaryotic CA's (see <PDOC00146>).

Hypothetical proteins yadF from Escherichia coli and HI1301 from Haemophilus influenzae also belong to this family.

We developed two signature patterns for this family of enzymes. Both patterns contain conserved residues that could be involved in binding zinc (cysteine and histidine).

Last update:

December 2004 / Pattern and text revised.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

PROK_CO2_ANHYDRASE_1, PS00704; Prokaryotic-type carbonic anhydrases signature 1  (PATTERN)

PROK_CO2_ANHYDRASE_2, PS00705; Prokaryotic-type carbonic anhydrases signature 2  (PATTERN)


1AuthorsGuilloton M.B. Korte J.J. Lamblin A.F. Fuchs J.A. Anderson P.M.
TitleCarbonic anhydrase in Escherichia coli. A product of the cyn operon.
SourceJ. Biol. Chem. 267:3731-3734(1992).
PubMed ID1740425

2AuthorsFukuzawa H. Suzuki E. Komukai Y. Miyachi S.
TitleA gene homologous to chloroplast carbonic anhydrase (icfA) is essential to photosynthetic carbon dioxide fixation by Synechococcus PCC7942.
SourceProc. Natl. Acad. Sci. U.S.A. 89:4437-4441(1992).
PubMed ID1584776

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