PROSITE documentation PDOC00612Protein secY signatures
The eubacterial secY protein [1] plays an important role in protein export. It interacts with the signal sequences of secretory proteins as well as with two other components of the protein translocation system: secA and secE. SecY is an integral plasma membrane protein of 419 to 492 amino acid residues that apparently contains ten transmembrane segments. Such a structure probably confers to secY a 'translocator' function, providing a channel for periplasmic and outer-membrane precursor proteins.
Homologs of secY are found in archaebacteria [2]. SecY is also encoded in the chloroplast genome of some algae [3] where it could be involved in a prokaryotic-like protein export system across the two membranes of the chloroplast endoplasmic reticulum (CER) which is present in chromophyte and cryptophyte algae.
In eukaryotes, the evolutionary related protein sec61-α plays a role in protein translocation through the endoplasmic reticulum; it is part of a trimeric complex that also consist of sec61-β and γ [4].
We have developed two signature patterns for secY proteins. The first corresponds to the second transmembrane region, which is the most conserved section of these proteins. The second spans the C-terminal part of the fourth transmembrane region, a short intracellular loop, and the N-terminal part of the fifth transmembrane region.
Last update:December 2004 / Pattern and text revised.
-------------------------------------------------------------------------------
PROSITE methods (with tools and information) covered by this documentation:
1 | Authors | Ito K. |
Title | SecY and integral membrane components of the Escherichia coli protein translocation system. | |
Source | Mol. Microbiol. 6:2423-2428(1992). | |
PubMed ID | 1406280 |
2 | Authors | Auer J. Spicker G. Boeck A. |
Title | Presence of a gene in the archaebacterium Methanococcus vannielii homologous to secY of eubacteria. | |
Source | Biochimie 73:683-688(1991). | |
PubMed ID | 1764515 |
3 | Authors | Douglas S.E. |
Title | A secY homologue is found in the plastid genome of Cryptomonas phi. | |
Source | FEBS Lett. 298:93-96(1992). | |
PubMed ID | 1544427 |
4 | Authors | Hartmann E. Sommer T. Prehn S. Goerlich D. Jentsch S. Rapoport T.A. |
Title | Evolutionary conservation of components of the protein translocation complex. | |
Source | Nature 367:654-657(1994). | |
PubMed ID | 8107851 | |
DOI | 10.1038/367654a0 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
View entry in original PROSITE document format
View entry in raw text format (no links)