Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00614WHEP-TRS domain signature and profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00614
A conserved domain of 46 amino acids has been shown [1] to exist in a number of higher eukaryote aminoacyl-transfer RNA synthetases. This domain is present one to six times in the following enzymes:
- Mammalian multifunctional aminoacyl-tRNA synthetase. The domain is present three times in a region that separates the N-terminal glutamyl-tRNA synthetase domain from the C-terminal prolyl-tRNA synthetase domain.
- Drosophila multifunctional aminoacyl-tRNA synthetase. The domain is present six times in the intercatalytic region.
- Mammalian tryptophanyl-tRNA synthetase. The domain is found at the N- terminal extremity.
- Mammalian, insect, nematode and plant glycyl-tRNA synthetase. The domain is found at the N-terminal extremity [2].
- Mammalian histidyl-tRNA synthetase. The domain is found at the N-terminal extremity.
The structure of a human WHEP-TRS domain has been solved by NMR (see <PDB:1FYJ>) [3]. It consists of two helices arranged in a helix-turn-helix. This domain may play a role in the association of tRNA-synthetases into multienzyme complexes [4].
We developed a signature pattern based on the first 29 positions of the WHEP-domain. We also developed a profile that covers the whole WHEP-TRS domain.
Last update:April 2006 / Pattern revised.
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PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Cerini C. Kerjan P. Astier M. Gratecos D. Mirande M. Semeriva M. |
| Title | A component of the multisynthetase complex is a multifunctional aminoacyl-tRNA synthetase. | |
| Source | EMBO J. 10:4267-4277(1991). | |
| PubMed ID | 1756734 |
| 2 | Authors | Nada S. Chang P.K. Dignam J.D. |
| Title | Primary structure of the gene for glycyl-tRNA synthetase from Bombyx mori. | |
| Source | J. Biol. Chem. 268:7660-7667(1993). | |
| PubMed ID | 8463296 |
| 3 | Authors | Jeong E.J. Hwang G.S. Kim K.H. Kim M.J. Kim S. Kim K.S. |
| Title | Structural analysis of multifunctional peptide motifs in human bifunctional tRNA synthetase: identification of RNA-binding residues and functional implications for tandem repeats. | |
| Source | Biochemistry 39:15775-15782(2000). | |
| PubMed ID | 11123902 | |
| DOI | 10.1021/bi001393h |
| 4 | Authors | Rho S.B. Lee J.S. Jeong E.J. Kim K.S. Kim Y.G. Kim S. |
| Title | A multifunctional repeated motif is present in human bifunctional tRNA synthetase. | |
| Source | J. Biol. Chem. 273:11267-11273(1998). | |
| PubMed ID | 9556618 |
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