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| PROSITE documentation PDOC00614 |
WHEP-TRS domain signature and profile
A conserved domain of 46 amino acids has been shown [1] to exist in a number of higher eukaryote aminoacyl-transfer RNA synthetases. This domain is present one to six times in the following enzymes:
- Mammalian multifunctional aminoacyl-tRNA synthetase. The domain is present three times in a region that separates the N-terminal glutamyl-tRNA synthetase domain from the C-terminal prolyl-tRNA synthetase domain.
- Drosophila multifunctional aminoacyl-tRNA synthetase. The domain is present six times in the intercatalytic region.
- Mammalian tryptophanyl-tRNA synthetase. The domain is found at the N- terminal extremity.
- Mammalian, insect, nematode and plant glycyl-tRNA synthetase. The domain is found at the N-terminal extremity [2].
- Mammalian histidyl-tRNA synthetase. The domain is found at the N-terminal extremity.
The structure of a human WHEP-TRS domain has been solved by NMR (see <PDB:1FYJ>) [3]. It consists of two helices arranged in a helix-turn-helix. This domain may play a role in the association of tRNA-synthetases into multienzyme complexes [4].
We developed a signature pattern based on the first 29 positions of the WHEP-domain. We also developed a profile that covers the whole WHEP-TRS domain.
Last update:April 2006 / Pattern revised.
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PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Cerini C. Kerjan P. Astier M. Gratecos D. Mirande M. Semeriva M. |
| Title | A component of the multisynthetase complex is a multifunctional aminoacyl-tRNA synthetase. | |
| Source | EMBO J. 10:4267-4277(1991). | |
| PubMed ID | 1756734 |
| 2 | Authors | Nada S. Chang P.K. Dignam J.D. |
| Title | Primary structure of the gene for glycyl-tRNA synthetase from Bombyx mori. | |
| Source | J. Biol. Chem. 268:7660-7667(1993). | |
| PubMed ID | 8463296 |
| 3 | Authors | Jeong E.J. Hwang G.S. Kim K.H. Kim M.J. Kim S. Kim K.S. |
| Title | Structural analysis of multifunctional peptide motifs in human bifunctional tRNA synthetase: identification of RNA-binding residues and functional implications for tandem repeats. | |
| Source | Biochemistry 39:15775-15782(2000). | |
| PubMed ID | 11123902 | |
| DOI | 10.1021/bi001393h |
| 4 | Authors | Rho S.B. Lee J.S. Jeong E.J. Kim K.S. Kim Y.G. Kim S. |
| Title | A multifunctional repeated motif is present in human bifunctional tRNA synthetase. | |
| Source | J. Biol. Chem. 273:11267-11273(1998). | |
| PubMed ID | 9556618 |
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