PROSITE documentation PDOC00621Glycosyl hydrolases family 3 active site
Description
It has been shown [1,2] that the following glycosyl hydrolases can be, on the basis of sequence similarities, classified into a single family:
- β glucosidases (EC 3.2.1.21) from the fungi Aspergillus wentii (A-3), Hansenula anomala, Kluyveromyces fragilis, Saccharomycopsis fibuligera, (BGL1 and BGL2), Schizophyllum commune and Trichoderma reesei (BGL1).
- β glucosidases from the bacteria Agrobacterium tumefaciens (Cbg1), Butyrivibrio fibrisolvens (bglA), Clostridium thermocellum (bglB), Escherichia coli (bglX), Erwinia chrysanthemi (bgxA) and Ruminococcus albus.
- Alteromonas strain O-7 β-hexosaminidase A (EC 3.2.1.52).
- Bacillus subtilis hypothetical protein yzbA.
- Escherichica coli hypothetical protein ycfO and HI0959, the corresponding Haemophilus influenzae protein.
One of the conserved regions in these enzymes is centered on a conserved aspartic acid residue which has been shown [3], in Aspergillus wentii β-glucosidase A3, to be implicated in the catalytic mechanism. We have used this region as a signature pattern.
Expert(s) to contact by email: Last update:April 2006 / Pattern revised.
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Technical section
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References
| 1 | Authors | Henrissat B. |
| Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. | |
| Source | Biochem. J. 280:309-316(1991). | |
| PubMed ID | 1747104 |
| 2 | Authors | Castle L.A. Smith K.D. Morris R.O. |
| Title | Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase gene involved in modifying a vir-inducing plant signal molecule. | |
| Source | J. Bacteriol. 174:1478-1486(1992). | |
| PubMed ID | 1537792 |
| 3 | Authors | Bause E. Legler G. |
| Title | Isolation and structure of a tryptic glycopeptide from the active site of beta-glucosidase A3 from Aspergillus wentii. | |
| Source | Biochim. Biophys. Acta 626:459-465(1980). | |
| PubMed ID | 6783081 |
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