PROSITE documentation PDOC00651
Prokaryotic transcription elongation factors signatures


Bacterial proteins greA and greB [1,2] are necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. Arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked DNA/RNA/ polymerase ternary complexes. Cleavage of the nascent transcript by cleavage factors, such as greA or greB, allows the resumption of elongation from the new 3'terminus. GreA induces cleavage 2 or 3 nucleotides behind the terminus while greB releases longer sequences up to 9 nucleotides in length.

GreA and greB are related proteins of about 160 amino-acid residues. We have developed two signature patterns for this protein family. The first corresponds to a conserved region in the N-terminal section, the second to a region in the C-terminal section.

Last update:

December 2004 / Pattern and text revised.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

GREAB_1, PS00829; Prokaryotic transcription elongation factors signature 1  (PATTERN)

GREAB_2, PS00830; Prokaryotic transcription elongation factors signature 2  (PATTERN)


1AuthorsBorukhov S. Sagitov V. Goldfarb A.
TitleTranscript cleavage factors from E. coli.
SourceCell 72:459-466(1993).
PubMed ID8431948

2AuthorsStebbins C.E. Borukhov S. Orlova M. Polyakov A. Goldfarb A. Darst S.A.
TitleCrystal structure of the GreA transcript cleavage factor from Escherichia coli.
SourceNature 373:636-640(1995).
PubMed ID7854424

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