PROSITE documentation PDOC00657Aspartyl proteases, omptin family signatures
Description
Escherichia coli protease VII (EC 3.4.21.87) (omptin) (gene ompT) [1] is an outer-membrane associated protease with a specificity for paired basic residues. It seems to be an aspartyl protease [2] inhibited by zinc ions. Omptin is highly similar to the following proteins:
- Salmonella typhimurium outer membrane protease E (gene pgtE) [3], also specific for paired basic residues.
- Yersinia pestis coagulase/fibrinolysin (plasminogen activator) (gene pla) [4]. This enzyme is encoded on a plasmid required for the pathogenic potential of the bacteria. It cleaves and activates plasminogen at the foci of infection; this allows plasmin to degrade fibrin barriers thus promoting the invasiveness of the plague bacteria.
- Escherichia coli outer membrane protease ompP [5].
The mature forms of these enzymes are proteins of about 280 to 300 amino-acid residues. As signature patterns, we selected two well conserved regions in the central part of these proteins.
Note:These proteins belong to family A26 in the classification of peptidases [6,E1].
Last update:January 2002 / Text revised.
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References
1 | Authors | Sugimura K. Nishihara T. |
Title | Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT. | |
Source | J. Bacteriol. 170:5625-5632(1988). | |
PubMed ID | 3056908 |
2 | Authors | Vandeputte-Rutten L. Kramer R.A. Kroon J. Dekker N. Egmond M.R. Gros P. |
Source | EMBO J. 20:5033-5039(2001). |
3 | Authors | Grodberg J. Dunn J.J. |
Title | Comparison of Escherichia coli K-12 outer membrane protease OmpT and Salmonella typhimurium E protein. | |
Source | J. Bacteriol. 171:2903-2905(1989). | |
PubMed ID | 2651422 |
4 | Authors | Sodeinde O.A. Goguen J.D. |
Title | Nucleotide sequence of the plasminogen activator gene of Yersinia pestis: relationship to ompT of Escherichia coli and gene E of Salmonella typhimurium. | |
Source | Infect. Immun. 57:1517-1523(1989). | |
PubMed ID | 2651310 |
5 | Authors | Kaufmann A. Stierhof Y.-D. Henning U. |
Title | New outer membrane-associated protease of Escherichia coli K-12. | |
Source | J. Bacteriol. 176:359-367(1994). | |
PubMed ID | 8288530 |
6 | Authors | Rawlings N.D. Barrett A.J. |
Title | Families of serine peptidases. | |
Source | Methods Enzymol. 244:19-61(1994). | |
PubMed ID | 7845208 |
E1 | Title | https://www.uniprot.org/docs/peptidas |
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