PROSITE documentation PDOC00669Signal peptidases II signature
Signal peptidases (SPases) [1] (also known as leader peptidases) remove the signal peptides from secretory proteins. In prokaryotes three types of SPases are known: type I (gene lepB) which is responsible for the processing of the majority of exported pre-proteins; type II (gene lsp) which only process lipoproteins, and a third type involved in the processing of pili subunits.
SPase II (EC 3.4.23.36), also known as lipoprotein signal peptidase, recognizes a conserved sequence and cuts in front of a cysteine residue to which a glyceride-fatty acid lipid is attached. SPase II is an integral membrane protein that is anchored in the cytoplasmic membrane.
There are a number of conserved regions in the sequence of Spase II from various bacteria [2], we have selected one of these regions as a signature pattern.
Note:These proteins belong to family A8 in the classification of peptidases [3,E1].
Expert(s) to contact by email: Last update:April 2006 / Pattern revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Dalbey R.E. Von Heijne G. |
| Title | Signal peptidases in prokaryotes and eukaryotes--a new protease family. | |
| Source | Trends Biochem. Sci. 17:474-478(1992). | |
| PubMed ID | 1455520 |
| 2 | Authors | Zhao X.J. Wu H.C. |
| Title | Nucleotide sequence of the Staphylococcus aureus signal peptidase II (lsp) gene. | |
| Source | FEBS Lett. 299:80-84(1992). | |
| PubMed ID | 1544479 |
| 3 | Authors | Rawlings N.D. Barrett A.J. |
| Title | Evolutionary families of metallopeptidases. | |
| Source | Methods Enzymol. 248:183-228(1995). | |
| PubMed ID | 7674922 |
| E1 | Title | https://www.uniprot.org/docs/peptidas |
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