|PROSITE documentation PDOC00686|
Acyl-CoA-binding protein (ACBP) is a small (10 Kd) protein that binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters . ACBP is also known as diazepam binding inhibitor (DBI) or endozepine (EP) because of its ability to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor .
ACBP is a highly conserved protein of about 90 residues that is found in all four eukaryotic kingdoms, Animalia, Plantae, Fungi and Protista, and in some eubacterial species .
Although ACBP occurs as a completely independent protein, intact ACB domains have been identified in a number of large, multifunctional proteins in a variety of eukaryotic species. These include large membrane-associated proteins with N-terminal ACB domains, multifunctional enzymes with both ACB and peroxisomal enoyl-CoA Delta(3), Delta(2)-enoyl-CoA isomerase domains, and proteins with both an ACB domain and ankyrin repeats (see <PDOC50088>) .
The ACB domain consists of four α-helices arranged in a bowl shape with a highly exposed acyl-CoA-binding site (see <PDB:1HBK>). The ligand is bound through specific interactions with residues on the protein, most notably several conserved positive charges that interact with the phosphate group on the adenosine-3'phosphate moiety, and the acyl chain is sandwiched between the hydrophobic surfaces of CoA and the protein .
ACBP belongs to a family that also includes:
As a signature pattern we selected the region that corresponds to residue 19 to 37 in mammalian ACBP. We also developed a profile which covers the entire ACB domain.Last update:
July 2006 / Text revised; profile added.
PROSITE methods (with tools and information) covered by this documentation:
|1||Authors||Rose T.M. Schultz E.R. Todaro G.J.|
|Title||Molecular cloning of the gene for the yeast homolog (ACB) of diazepam binding inhibitor/endozepine/acyl-CoA-binding protein.|
|Source||Proc. Natl. Acad. Sci. U.S.A. 89:11287-11291(1992).|
|2||Authors||Costa E. Guidotti A.|
|Title||Diazepam binding inhibitor (DBI): a peptide with multiple biological actions.|
|Source||Life Sci. 49:325-344(1991).|
|3||Authors||Burton M. Rose T.M. Faergeman N.J. Knudsen J.|
|Title||Evolution of the acyl-CoA binding protein (ACBP).|
|Source||Biochem. J. 392:299-307(2005).|
|4||Authors||van Aalten D.M.F. Milne K.G. Zou J.Y. Kleywegt G.J. Bergfors T. Ferguson M.A.J. Knudsen J. Jones T.A.|
|Title||Binding site differences revealed by crystal structures of Plasmodium falciparum and bovine acyl-CoA binding protein.|
|Source||J. Mol. Biol. 309:181-192(2001).|
|5||Authors||Pusch W. Balvers M. Hunt N. Ivell R.|
|Title||A novel endozepine-like peptide (ELP) is exclusively expressed in male germ cells.|
|Source||Mol. Cell. Endocrinol. 122:69-80(1996).|
|6||Authors||Suk K. Kim Y.-H. Hwang D.-Y. Ihm S.H. Yoo H.J. Lee M.S.|
|Title||Molecular cloning and expression of a novel human cDNA related to the diazepam binding inhibitor.|
|Source||Biochim. Biophys. Acta 1454:126-131(1999).|