PROSITE documentation PDOC00688
Nickel-dependent hydrogenases b-type cytochrome subunit signatures


Bacterial membrane-bound nickel-dependent hydrogenases [1,2,3] seem to be associated with a b-type cytochrome involved in electron transfer from hydrogen to oxygen. This cytochrome is a protein of about 28 Kd that seems to have four transmembrane regions. The gene coding for this cytochrome is adjacent to that coding for the large subunit of the hydrogenase. It has been assigned a variety of names in different species: hupC, hyaC, hydC or hoxZ.

We developed two signature patterns for this protein. The first corresponds to the first potential transmembrane region. The second signature includes the C-terminal part of a hydrophilic loop between the third and the fourth transmembrane region as well as the first half of the fourth transmembrane region. Both signatures contain conserved histidines that could be involved in binding the heme iron group.

Last update:

May 2004 / Text revised.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

NI_HGENASE_CYTB_1, PS00882; Nickel-dependent hydrogenases b-type cytochrome subunit signature 1  (PATTERN)

NI_HGENASE_CYTB_2, PS00883; Nickel-dependent hydrogenases b-type cytochrome subunit signature 2  (PATTERN)


1AuthorsCauvin B. Colbeau A. Vignais P.M.
TitleThe hydrogenase structural operon in Rhodobacter capsulatus contains a third gene, hupM, necessary for the formation of a physiologically competent hydrogenase.
SourceMol. Microbiol. 5:2519-2527(1991).
PubMed ID1791762

2AuthorsDross F. Geisler V. Lenger R. Theis F. Krafft T. Fahrenholz F. Kojro E. Duchene A. Tripier D. Juvenal K.
TitleThe quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes.
SourceEur. J. Biochem. 206:93-102(1992).
PubMed ID1587288

3AuthorsHidalgo E. Palacios J.M. Murillo J. Ruiz-Argueso T.
TitleNucleotide sequence and characterization of four additional genes of the hydrogenase structural operon from Rhizobium leguminosarum bv. viciae.
SourceJ. Bacteriol. 174:4130-4139(1992).
PubMed ID1597428

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