PROSITE documentation PDOC00688Nickel-dependent hydrogenases b-type cytochrome subunit signatures
Bacterial membrane-bound nickel-dependent hydrogenases [1,2,3] seem to be associated with a b-type cytochrome involved in electron transfer from hydrogen to oxygen. This cytochrome is a protein of about 28 Kd that seems to have four transmembrane regions. The gene coding for this cytochrome is adjacent to that coding for the large subunit of the hydrogenase. It has been assigned a variety of names in different species: hupC, hyaC, hydC or hoxZ.
We developed two signature patterns for this protein. The first corresponds to the first potential transmembrane region. The second signature includes the C-terminal part of a hydrophilic loop between the third and the fourth transmembrane region as well as the first half of the fourth transmembrane region. Both signatures contain conserved histidines that could be involved in binding the heme iron group.
Last update:May 2004 / Text revised.
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PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Cauvin B. Colbeau A. Vignais P.M. |
| Title | The hydrogenase structural operon in Rhodobacter capsulatus contains a third gene, hupM, necessary for the formation of a physiologically competent hydrogenase. | |
| Source | Mol. Microbiol. 5:2519-2527(1991). | |
| PubMed ID | 1791762 |
| 2 | Authors | Dross F. Geisler V. Lenger R. Theis F. Krafft T. Fahrenholz F. Kojro E. Duchene A. Tripier D. Juvenal K. |
| Title | The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes. | |
| Source | Eur. J. Biochem. 206:93-102(1992). | |
| PubMed ID | 1587288 |
| 3 | Authors | Hidalgo E. Palacios J.M. Murillo J. Ruiz-Argueso T. |
| Title | Nucleotide sequence and characterization of four additional genes of the hydrogenase structural operon from Rhizobium leguminosarum bv. viciae. | |
| Source | J. Bacteriol. 174:4130-4139(1992). | |
| PubMed ID | 1597428 |
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