PROSITE documentation PDOC00701Glutamate 5-kinase signature
Glutamate 5-kinase (EC 2.7.2.11) (γ-glutamyl kinase) (GK) is the enzyme that catalyzes the first step in the biosynthesis of proline from glutamate, the ATP-dependent phosphorylation of L-glutamate into L-glutamate 5-phosphate.
In eubacteria (gene proB) and yeast [1] (gene PRO1), GK is a monofunctional protein, while in plants and mammals, it is a bifunctional enzyme (P5CS) [2] that consists of two domains: a N-terminal GK domain and a C-terminal γ-glutamyl phosphate reductase domain (EC 1.2.1.41) (see <PDOC00940>).
As a signature pattern, we selected a highly conserved glycine-and alanine-rich region located in the central section of these enzymes.
Yeast hypothetical protein YHR033w is highly similar to GK.
Last update:December 2004 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Li W. Brandriss M.C. |
| Title | Proline biosynthesis in Saccharomyces cerevisiae: molecular analysis of the PRO1 gene, which encodes gamma-glutamyl kinase. | |
| Source | J. Bacteriol. 174:4148-4156(1992). | |
| PubMed ID | 1350780 |
| 2 | Authors | Hu C.-A.A. Delauney A.J. Verma D.P.S. |
| Title | A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 89:9354-9358(1992). | |
| PubMed ID | 1384052 |
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