PROSITE documentation PDOC00940Gamma-glutamyl phosphate reductase signature
γ-glutamyl phosphate reductase (EC 1.2.1.41) (GPR) is the enzyme that catalyzes the second step in the biosynthesis of proline from glutamate, the NADP-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate.
In eubacteria (gene proA) and yeast [1] (gene PRO2), GPR is a monofunctional protein, while in plants and mammals, it is a bifunctional enzyme (P5CS) [2] that consists of two domains: a N-terminal glutamate 5-kinase domain (EC 2.7.2.11) (see <PDOC00701>) and a C-terminal GPR domain.
As a signature pattern, we selected a conserved region that contains two histidine residues. This region is located in the last third of GPR.
Last update:April 2006 / Pattern revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Pearson B.M. Hernando Y. Payne J. Wolf S.S. Kalogeropoulos A. Schweizer M. |
| Title | Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII. | |
| Source | Yeast 12:1021-1031(1996). | |
| PubMed ID | 8896266 | |
| DOI | 10.1002/(SICI)1097-0061(199609)12:10B<1021::AID-YEA981>3.0.CO;2-7 |
| 2 | Authors | Hu C.-A.A. Delauney A.J. Verma D.P.S. |
| Title | A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 89:9354-9358(1992). | |
| PubMed ID | 1384052 |
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