PROSITE documentation PDOC00771DnaA protein signature
The bacterial dnaA protein [1,2,3] plays an important role in initiating and regulating chromosomal replication. DnaA is an ATP- and DNA-binding protein. It binds specifically to 9 bp nucleotide repeats known as dnaA boxes which are found in the chromosome origin of replication (oriC).
DnaA is a protein of about 50 Kd that contains two conserved regions: the first is located in the N-terminal half and corresponds to the ATP-binding domain, the second is located in the C-terminal half and could be involved in DNA-binding. We used the most conserved part of the putative DNA-binding domain as a signature pattern.
Last update:December 2004 / Pattern and text revised.
-------------------------------------------------------------------------------
PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Skarstad K. Boye E. |
| Title | The initiator protein DnaA: evolution, properties and function. | |
| Source | Biochim. Biophys. Acta 1217:111-130(1994). | |
| PubMed ID | 8110826 |
| 2 | Authors | Yoshikawa H. Ogasawara N. |
| Title | Structure and function of DnaA and the DnaA-box in eubacteria: evolutionary relationships of bacterial replication origins. | |
| Source | Mol. Microbiol. 5:2589-2597(1991). | |
| PubMed ID | 1779750 |
| 3 | Authors | Georgopoulos C. |
| Title | The E. coli dnaA initiation protein: a protein for all seasons. | |
| Source | Trends Genet. 5:319-321(1989). | |
| PubMed ID | 2558436 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
View entry in original PROSITE document format
View entry in raw text format (no links)