PROSITE documentation PDOC00790RNA polymerases M / 15 Kd subunits signature
In eukaryotes, there are three different forms of DNA-dependent RNA polymerases (EC 2.7.7.6) transcribing different sets of genes. Each class of RNA polymerase is an assemblage of ten to twelve different polypeptides. In archaebacteria, there is generally a single form of RNA polymerase which also consist of an oligomeric assemblage of 10 to 13 polypeptides.
It has recently been shown [1,2] that small subunits of about 15 Kd, found in polymerase types I and II, are highly conserved. Subunits known to belong to this family are:
- Yeast RPA12 subunit from RNA polymerases I.
- Yeast RPB9 subunit from RNA polymerase II.
- Yeast RPC11 subunit from RNA polymerase III.
- Mammalian RPB14.5 (gene POLR2I) from RNA polymerase II.
- Drosophila RpII15 from RNA polymerase II.
- Archebacterial RNA polymerase subunit M (gene rpoM).
These proteins contain a probable zinc finger in their N-terminal region and a C-terminal zinc ribbon domain (see <PDOC00383>). As a signature pattern for these subunits, we selected the region that contains the zinc finger.
Last update:February 2024 / Pattern revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Acker J. Wintzerith M. Vigneron M. Kedinger C. |
| Title | Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II. | |
| Source | Nucleic Acids Res. 21:5345-5350(1993). | |
| PubMed ID | 8265347 |
| 2 | Authors | Nogi Y. Yano R. Dodd J. Carles C. Nomura M. |
| Title | Gene RRN4 in Saccharomyces cerevisiae encodes the A12.2 subunit of RNA polymerase I and is essential only at high temperatures. | |
| Source | Mol. Cell. Biol. 13:114-122(1993). | |
| PubMed ID | 8417319 |
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