PROSITE documentation PDOC00809NAD-dependent DNA ligase signatures
DNA ligase (polydeoxyribonucleotide synthase) is the enzyme that joins two DNA fragments by catalyzing the formation of an internucleotide ester bond between phosphate and deoxyribose. It is active during DNA replication, DNA repair and DNA recombination. There are two forms of DNA ligase: one requires ATP (EC 6.5.1.1), the other NAD (EC 6.5.1.2).
Bacterial DNA ligases are NAD-dependent. They are proteins of about 75 to 85 Kd whose sequence is well conserved [1]. They also show similarity to yicF, an Escherichia coli hypothetical protein of 63 Kd. We developed two signature patterns for this family of proteins; these signatures are based on conserved regions in the N-terminal half.
Last update:December 2004 / Patterns and text revised.
-------------------------------------------------------------------------------
PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Shark K.B. Conway T. |
| Title | Cloning and molecular characterization of the DNA ligase gene (lig) from Zymomonas mobilis. | |
| Source | FEMS Microbiol. Lett. 75:19-26(1992). | |
| PubMed ID | 1526462 |
| 2 | Authors | Gentry D. Bengra C. Ikehara K. Cashel M. |
| Title | Guanylate kinase of Escherichia coli K-12. | |
| Source | J. Biol. Chem. 268:14316-14321(1993). | |
| PubMed ID | 8390989 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
View entry in original PROSITE document format
View entry in raw text format (no links)