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PROSITE documentation PDOC00821 |
A family of bacterial and eukaryotic endonucleases (EC 3.1.30.-) share the following characteristics: they act on both DNA and RNA, cleave double-stranded and single-standed nucleic acids and require a divalent ion such as magnesium for their activity. The enzymes that belongs to this family are:
An histidine has been shown [1] to be essential for the activity of the Serratia marcescens nuclease. This residue is located in a conserved region which can be used as a signature pattern and which also contains an aspartic acid residue that could be implicated in the binding of the divalent ion.
Expert(s) to contact by email: Last update:July 1999 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
1 | Authors | Friedhoof P. Gimadutdinow O. Pingoud A. |
Title | Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis. | |
Source | Nucleic Acids Res. 22:3280-3287(1994). | |
PubMed ID | 8078761 |