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PROSITE documentation PDOC00877Glycosyl hydrolases family 45 active site
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00877
The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family K or as the glycosyl hydrolases family 45 [3,E1]. The enzymes which are currently known to belong to this family are listed below.
- Endoglucanase 5 from Humicola insolens.
- Endoglucanase 5 from Trichoderma reesei (egl5).
- Endoglucanase K from Fusarium oxysporum.
- Endoglucanase B from Pseudomonas fluorescens (celB).
- Endoglucanase 1 from Ustilago maydis (egl1).
The best conserved regions in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown [4] to act as a nucleophile in the catalytic mechanism. We use this region as a signature pattern.
Expert(s) to contact by email: Last update:November 1997 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Beguin P. |
| Title | Molecular biology of cellulose degradation. | |
| Source | Annu. Rev. Microbiol. 44:219-248(1990). | |
| PubMed ID | 2252383 | |
| DOI | 10.1146/annurev.mi.44.100190.001251 |
| 2 | Authors | Gilkes N.R. Henrissat B. Kilburn D.G. Miller R.C. Jr. Warren R.A.J. |
| Title | Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. | |
| Source | Microbiol. Rev. 55:303-315(1991). | |
| PubMed ID | 1886523 |
| 3 | Authors | Henrissat B. Bairoch A. |
| Title | New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. | |
| Source | Biochem. J. 293:781-788(1993). | |
| PubMed ID | 8352747 |
| 4 | Authors | Davies G.J. Dodson G.G. Hubbard R.E. Tolley S.P. Dauter Z. Wilson K.S. Hjort C. Mikkelsen J.M. Rasmussen G. Schuelein M. |
| Title | Structure and function of endoglucanase V. | |
| Source | Nature 365:362-364(1993). | |
| PubMed ID | 8377830 | |
| DOI | 10.1038/365362a0 |
| E1 | Title | https://www.uniprot.org/docs/glycosid |
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