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PROSITE documentation PDOC00927
Glypicans signature

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PURL: https://purl.expasy.org/prosite/documentation/PDOC00927

Description

Glypicans [1,2] are a family of heparan sulfate proteoglycans which are anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Structurally, these proteins consist of three separate domains:

 a) A signal sequence;
 b) An  extracellular  domain of about 500 residues that contains 12 conserved
    cysteines probably involved in disulfide bonds and which also contains the
    sites of attachment of the heparan sulfate glycosaminoglycan side chains;
 c) A  C-terminal  hydrophobic  region  which  is post-translationally removed
    after formation of the GPI-anchor.

The proteins known to belong to this family are:

Glypican 1 (GPC1).
Glypican 2 (GPC2) or cerebroglycan.
Glypican 3 (GPC3) or OCI-5. In man, defects in GPC3 are the cause of a X- linked genetic disease, Simpson-Galabi-Behmel syndrome (SGBS).
Glypican 4 (GPC4) or K-glypican.
Glypican 5 (GPC5).
Glypican 6 (GPC6).
Drosophila protein dally.

The signature pattern that we developed for glypicans is located in the central section of the extracellular domain and contains five of the conserved cysteines.

Last update:

April 2006 / Pattern revised.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

GLYPICAN, PS01207; Glypicans signature (PATTERN)

Consensus pattern:
C-x(2)-C-x-[GS]-[LIVM]-x(4)-P-C-x(2)-[FY]-C-x(2)-[LIVM]-x(2)-G-C
The C's are probably involved in a disulfide bonds
Sequences in UniProtKB/Swiss-Prot known to belong to this class: 21
- detected by PS01207: 20 (true positives)
- undetected by PS01207: 1 (1 false negative and 0 'partial')
Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01207:
NONE.
Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
Clustal format, color, condensed view / Clustal format, color / Clustal format, plain text / Fasta format
Retrieve the sequence logo from the alignment
Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01207
Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01207
Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01207
Matching PDB structures: pdb_00004acr pdb_00004ad7 pdb_00004bwe pdb_00004ywt ... [ALL]

References

1	Authors	Weksberg R. Squire J.A. Templeton D.M.
	Title	Glypicans: a growing trend.
	Source	Nat. Genet. 12:225-227(1996).
	PubMed ID	8589707
	DOI	10.1038/ng0396-225

2	Authors	Watanabe K. Yamada H. Yamaguchi Y.
	Title	K-glypican: a novel GPI-anchored heparan sulfate proteoglycan that is highly expressed in developing brain and kidney.
	Source	J. Cell Biol. 130:1207-1218(1995).
	PubMed ID	7657705

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PROSITE documentation PDOC00927Glypicans signature

PROSITE documentation PDOC00927
Glypicans signature